2013 Fiscal Year Final Research Report
Cancer stem cells in colorectal cancer and the prognostic significance markers
| Project/Area Number |
23591872
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
ZHENG Yun-Wen 横浜市立大学, 医学部, 助教 (80404995)
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| Research Collaborator |
TANIGUCHI Hideki 横浜市立大学, 医学研究科, 教授 (70292555)
TAKABE Takanori 横浜市立大学, 医学部, 准教授 (20612625)
KOZAWA Mayumi 横浜市立大学, 医学研究科, 大学院生
GUAN Hong-bin 横浜市立大学, 医学研究科, 大学院生
KONDO Akihisa 横浜市立大学, 医学研究科, 大学院生
OTA Mitsuyoshi 横浜市立大学, 市民総合医療センター, 准教授 (60315794)
TATSUMI Kenji 横浜市立大学, 医学部, 助教 (80567628)
ISHIKAWA Yasushi 横浜市立大学, 医学研究科, 准教授 (70254208)
ENDO Itaru 横浜市立大学, 医学研究科, 教授 (60211091)
OSHIMA Takashi 横浜市立大学, 市民総合医療センター, 准教授 (10448665)
FUJII Shoichi 横浜市立大学, 市民総合医療センター, 准教授 (70326065)
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| Project Period (FY) |
2011 – 2013
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| Keywords | CD44variant2 / 予後予測因子 / 大腸癌 / 癌幹細胞 / CD133 |
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| Research Abstract |
CD133 and CD44 are putative cancer stem cell markers in colorectal cancer (CRC). However, their clinical significance is currently unclear. In this study, we evaluated primary CRC cell to determine the significance of several cancer stem cell markers, including CD133 and CD44, as predictors of tumourigenesis and prognosis. We fund that CD44+, CD133+ and CD133+CD44+ sub-populations were significantly more tumourigenic than the total cell population. The clinical samples expressed several transcript variants of CD44. Variant 2 was specifically overexpressed in both primary tumours and xenografts in comparison with the normal mucosa. A prognostic assay using qRT-PCR showed that the CD44v2high group had a significantly worse prognosis than the CD44v2low group.
In conlusion, CD44 is an important CSC marker in CRC patients. And the CRC patients with high expression of CD44v2 have a poorer prognosis than patients with other CD44 variants.
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Research Products
(39 results)
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[Journal Article] Prognostic significance of CD44 variant 2 upregulation in colorectal cancer2014
Author(s)
Ozawa M, Ichikawa Y, Zheng YW, Oshima T, Miyata H, Nakazawa K, Guan HB, Shiozawa M, Akaike M, Watanabe K, Ota M, Fujii S, Kunisaki C, Ishikawa T, Tanaka K, Akiyama H, Endo I, and Taniguchi H
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Journal Title
Br J Cancer
Volume: (in press)
DOI
Peer Reviewed
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[Journal Article] The CD133+CD44+ Precancerous Subpopulation of Oval Cells is a Therapeutic Target for Hepatocellular Carcinoma2014
Author(s)
Zheng YW, Tsuchida T, Shimao T, Li B, Takebe T, Zhang RR, Sakurai Y, Ueno Y, Sekine K, Ishibashi N, Imajima M, Tanaka T, and Taniguchi H
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Journal Title
Stem Cells Dev
Volume: (In press)
DOI
Peer Reviewed
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[Journal Article] Ring1B Promotes Hepatic Stem/Progenitor Cell Expansion via Simultaneous Suppression of Cdkn1a and Cdkn2a2014
Author(s)
Koike H, Ueno Y, Naito T, Shiina T, Nakata S, Ouchi R, Obana Y, Sekine K, Zheng YW, Takebe T, Isono K, Koseki H, Taniguchi H
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Journal Title
Hepatology
Volume: (Epub ahead of print)
DOI
Peer Reviewed
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[Journal Article] The evidence of sophisticatedly heterogeneous population of human umbilical vein endothelial cells2014
Author(s)
Zheng YW, Nie YZ, Tsuchida T, Zhang RR, Aoki K, Sekine K, Ogawa M, Takebe T, Ueno Y, Sakakibara H, Hirahara F, Taniguchi H
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Journal Title
Transplant Proc
Volume: 46
Pages: 1251-3
DOI
Peer Reviewed
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