2013 Fiscal Year Final Research Report
Basic research of mechanism of malignant pheochromocytoma
| Project/Area Number |
23591889
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | University of Tsukuba |
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Principal Investigator |
HARA Hisato 筑波大学, 医学医療系, 教授 (80189688)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEKOSHI Kazuhiro 筑波大学, 医学医療系, 教授 (40261804)
ISHII Kiyoaki 筑波大学, 医学医療系, 助教 (80419150)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 褐色細胞腫 / mTOR |
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| Research Abstract |
This research was performed to explore the malignant potential of phechromocytoma and the mechanism of effect of molecular targetted therapy. We examined that Rat pheochromocytoma cell line PC12 was treated with mTOR inhibitor and Sunitinib. Antiproliferative effect and the increase of apoptosis effect was showed.
We demonstrated that sunitinib significantly increased the levels of LC3-II.Following sunitinib treatment, immunofluorescent imaging revealed a marked increased punctate LC3-II distribution.Inhibition of autophagy by siRNAs targeting Atg13 or by pharmacological inhibition with ammonium chloride, enhanced both sunitinib-induced apoptosis and anti-proliferation. Inhibition of autophagy may be a promising therapeutic option for improving the anti-tumor effect of sunitinib.
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[Journal Article] Identical germline mutations in the TMEM127 gene in 2 unrelated Japanese patients with bilateral pheochromocytoma2012
Author(s)
Naomi Takeichi, Sanae Midorikawa, Atsushi Watanabe, Banyar Than Naing, Hideki Tamura, Toshiko Kano, Hitoshi Sugihara, Sumiko Nissato, Yuria Saito, Yuichi Aita, Kiyo-aki Ishii, Takehito Igarashi, Yasushi Kawakami, Hisato Hara, Tatsuhiko Ikeda, Kazuo Shimizu, Shinichi Suzuki, Hitoshi Shimano, Masashi Kawamoto, Takashi Shimada, Tsuyoshi Watanabe, Shinichi Oikawa, Kazuhiro Takekoshi
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Journal Title
DOI
Peer Reviewed
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