2013 Fiscal Year Final Research Report
Novel therapeutic starategy for CEACAM1 expressing colorectal cancer cells beyond the invasion front
| Project/Area Number |
23591904
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Wakayama Medical University |
|---|
Principal Investigator |
YOKOYAMA Shozo 和歌山県立医科大学, 医学部, 講師 (90398462)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HOTTA Tsukasa 和歌山県立医科大学, 医学部, 講師 (50244744)
TAKIFUJI Katsunari 和歌山県立医科大学, 医学部, 講師 (00254540)
MATSUDA Kenji 和歌山県立医科大学, 医学部, 助教 (30398458)
YAMAUE Hiroki 和歌山県立医科大学, 医学部, 教授 (20191190)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | CEACAM1 / 大腸癌 / 癌幹細胞様特性 / 抗癌剤感受性 |
|---|
| Research Abstract |
Enhanced CEACAM-4S (short cytoplasmic isoform) induced CD44 expression, incresed ALDH1 activity, and anchorage indeoendent growth of colorectal cancer cells compared with vector control, suggesting that a possibility that CEACAM1-4S induce CSC property. In clinical setting, Overexpression of CEACAM1-S (short cytoplasmic isoform) at the invasion fronat of colorectal cancer tissue indicated poor survival of patients. By addition of 5FU to colorectal cancer cells on plastic, CEACAM1-4L transfected HT29 cells significantly survived in comparison with CEACAM1-4S or vector control transfected cells. In 3D culture, addition of 5FU induced apoptotic burst of colorectal cancer sphere formed by CEACAM1-4S. A multivariate analysis for recurrence after curative surgery and adjuvant chemotherapy for Stage III colorectal cancer patients showed that the hollow spheroid formation beyond the invasive front is an independent risk factor.
|
-
[Journal Article] CEACAM1 Long Cytoplasmic Domain Isoform is Associated with Invasion and Recurrence of Hepatocellular Carcinoma2014
Author(s)
Kiriyama S, Yokoyama S, Ueno M, Hayami S, Ieda J, Yamamoto N, Yamaguchi S, Mitani Y, Nakamura Y, Tani M, Mishra L, Shively JE, Yamaue H
-
Journal Title
Ann Surg Oncol
Volume: (Epub ahead of print)
Peer Reviewed
-
[Journal Article] Hollow spheroids beyond the invasive margin indicate the malignant potential of colorectal cancer2011
Author(s)
Tamura K, Yokoyama S, Ieda J, Takifuji K, Hotta T, Matsuda K, Oku Y, Watanabe T, Nasu T, Kiriyama S, Yamamoto N, Nakamura Y, Shively JE, Yamaue H
-
Journal Title
BMJ Open
Volume: 1(1)
Pages: e000179
DOI
Peer Reviewed
-
[Journal Article] Re-expression of CEACAM1 long cytoplasmic domain isoform is associated with invasion and migration of colorectal cancer2011
Author(s)
Ieda J, Yokoyama S, Tamura K, Takifuji K, Hotta T, Matsuda K, Oku Y, Nasu T, Kiriyama S, Yamamoto N, Nakamura Y, Shively JE, Yamaue H
-
Journal Title
Int J Cancer
Volume: 129(6)
Pages: 1351-61
DOI
Peer Reviewed
-
-
[Presentation] Hollow spheroids beyond the invasive front indicates the malignant potential of colorectal cancer2014
Author(s)
Shozo Yokoyama, Junji Ieda, Naoyuki Yamamoto, Yasuyuki Mitani, Yuki Mizumoto, Katsunari Takifuji, Tsukasa Hotta, Kenji Matsuda, Takashi Watanabe, Masaji Tani and Hiroki Yamaue
Organizer
2014 GI Cancer Symposium
Place of Presentation
San Francisco, USA
Year and Date
2014-01-18
-
-
-
[Presentation] CEACAM1 long cytoplasmic domain isoform is associated with invasion and recurrence of hepatocellular carcinoma2013
Author(s)
Shozo Yokoyama, Shigehisa Kiriyama, Masaki Ueno, Shinya Hayami, Junji Ieda,Naoyuki Yamamoto, Shunsuke Yamaguchi,Yasuyuki Mitani, Yasushi Nakamura, Masaji Tani1 Lopa Mishra, John E. Shively, and Hiroki Yamaue
Organizer
23th International CEA Symposium
Place of Presentation
Ohio, USA
Year and Date
2013-08-16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
