Analysis of novel Hedgehog inhibitors for esophageal cancer

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591924
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Nagoya University
• Principal Investigator: FUKAYA MASAHIDE 名古屋大学, 医学部附属病院, 病院講師 (10420382)
• Co-Investigator(Kenkyū-buntansha): NAGINO Masato 名古屋大学, 医学系研究科, 教授 (20237564) ; YOKOYAMA Yukihiro 名古屋大学, 医学部附属病院, 講師 (80378091) ; KOKURYO Toshio 名古屋大学, 医学系研究科, 特任講師 (60378023)
• Project Period (FY): 2011 – 2013
• Keywords: 食道癌 / Hedgehog / 相互マシンラーニング法

Research Abstract

Several chemical compounds were identified for cancer therapy using the machine learning method. These compounds inhibited the proliferation and induced the cell death in cancer cell lines. The chemical compound suppressed the tumor growth in the xenograft mouse mode. As the tumor did not grow up in the femoral region of nude mouse, in vivo cell strain was not established from the esophagus cancer tissue. We challenged the establishment of in vivo cell strain using other cancer cell lines. However it was not able to establish using even pancreatic cancer. In the collaborative institute, in vivo cell strain was established in SCID mouse using the colon cancer tissue. Furthermore, DNA array revealed the expression of lincRNA (long intergenic noncoding RNA) in the mouse tumor treated by chemical compound. This data suggested that lincRNA was related to the mechanism of the chemical compound.