Selective neural differentiation method using ES gut

2013 Fiscal Year Final Research Report

• Project/Area Number: 23591969
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Nara Medical University
• Principal Investigator: MISAWA Hiromi 奈良県立医科大学, 医学部, その他 (50281275)
• Co-Investigator(Kenkyū-buntansha): TAKAKI Miyako 奈良県立医科大学, 医学部, 特任教授 (00033358) ; MATSUYOSHI Hiroko 奈良県立医科大学, 医学部, 助教 (10448772)
• Project Period (FY): 2011 – 2013
• Keywords: 胚性幹細胞 / ES腸管 / セロトニン４受容体 / 腸壁内神経

Research Abstract

From mouse embryonic stem (ES) cells, the gut-like organ expressing peristalsis-like movements could be stably formed. In this gut, pacemaker cells (ICC) generating peristalsis-like movements could be stably differentiated without any differentiation inducing factors.
In the present study, during the process forming the ES gut, a special neuron differentiation inducing factor (serotonin 4 receptor agonist) was applied, succeeding in formation of ES guts expressing more perfect peristalses.
The results of this study would provide many hints to clarify the conditions and explore therapies of bowel diseases lacking enteric neurons.

Research Products

• [Journal Article] In vivo imaging of enteric neurogenesis in the deep tissue of mouse small intestine (2013)
  • Author(s): Goto K, Kato G, Kawahara I, Luo Y, Obata K, Misawa H, Ishikawa T, Kuniyasu H, Nabekura J, Takaki M.
  • Journal Title: PLoS Volume: 8(1) Pages: e54814
  • DOI: 10.1371/journal.pone.0054814
  • Peer Reviewed
• [Journal Article] In vitro morphological bud formation in organ-like three-dimensional structure from mouse ES cells induced by FGF10 signaling (2012)
  • Author(s): Ohbuchi T, Takaki M, Misawa H, Suzuki H, Ueta Y.
  • Journal Title: Commun Integr Biol Volume: 5(4) Pages: 1-4
  • DOI: 10.4161/cib.20093
  • Peer Reviewed
• [Journal Article] The comparison of effects of a selective 5-HT reuptake inhibitor versus a 5-HT4 receptor agonist on in vivo neurogenesis at the rectal anasotomosis in rats (2012)
  • Author(s): Kawahara I, Kuniyasu H, Matsuyoshi H, Goto K, Obata K, Misawa H, Fujii H, Takaki M.
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 302 Pages: G588-G597
  • DOI: 10.1152/ajpgi.00284.2011
  • Peer Reviewed
• [Journal Article] In vitro enhanced differentiation of neural networks in ESgut-like organ from mouse ES cells by a 5-BT4-receptor activation (2011)
  • Author(s): Takaki M, Misawa H, Matsuyoshi H, Kawahara I, Goto K, Zhang GX, Obata K, Kuniyasu B.
  • Journal Title: Biochem Biophys Res Commun Volume: 406 Pages: 529-533
  • DOI: 10.1016/j.bbrc.2011.02.072
  • Peer Reviewed
• [Presentation] 5-HT4受容体居性化を介したin vitro神経分化促進作用とin vivo神経再生・新生促進作用 (シンポジウム消化器疾患における5-HT受容体を標的にした新たな創薬展開) (2012)
  • Author(s): 高木 都, 三澤 裕美, 松吉 ひろ子, 川原 勲, 後藤 桂, 小畑 孝二, 國安弘基
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 国立京都国際会館京都
  • Year and Date: 20120314-16
• [Presentation] 選択的セロトニン再取り込み阻害薬fluvoxamine(SSRI)のin vitro神経分化,in vivo神経再生・新生作用に対する効果:セロトニン4受容体刺激薬との比較 (2011)
  • Author(s): 高木 都, 川原 勲, 三澤裕美, 後藤桂, 松吉ひろ子, 小畑孝二, 國安弘基
  • Organizer: 第13回日本神経消化器病学会
  • Place of Presentation: 栃木総合文化センター栃木
  • Year and Date: 2011-11-05
• [Presentation] ES gut-like organ (2011)
  • Author(s): Takaki M, Misawa H, Matsuyoshi H, Kuniyasu H.
  • Organizer: An annual meeting of The Korean Society of Smooth Muscle Research in Seoul National University Bundang Hospital
  • Year and Date: 2011-06-18