To explore miR-195 mechanisms of acquired resistance against TMZ in glioblastoma.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592095
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Nagasaki University
• Principal Investigator: UJIFUKU Kenta 長崎大学, 医歯(薬)学総合研究科, 客員研究員 (20437867)
• Co-Investigator(Kenkyū-buntansha): MATSUO Takayuki 長崎大学, 大学院医歯薬学総合研究科展開医療科学講座脳神経外科学, 准教授 (00274655) ; HAYASHI Kentaro 長崎大学, 大学院医歯薬学総合研究科展開医療科学講座脳神経外科学, 講師 (40404222) ; KAMADA Kensaku 長崎大学, 大学院医歯薬学総合研究科展開医療科学講座脳神経外科学, 助教 (30549655)
• Co-Investigator(Renkei-kenkyūsha): MITSUTAKE Norisato 長崎大学, 原爆後障害医療研究所 社会医学部門放射線災害医療研究分野, 准教授 (50404215)
• Project Period (FY): 2011 – 2013
• Keywords: 脳腫瘍 / 神経膠芽腫 / 脳血管関門 / FGF-2 / Niche / 脳血管関門モデル

Research Abstract

The initial purpose of this study was to explore miR-195 mechanisms of acquired resistance against TMZ in glioblastoma (GBM). Unexpected results have been obtained and they have not been published yet.
Alternative study approach has been performed and intriguing results have been obtained. This study was examined to explore the mechanism of invasion front niche of GBM. Using new in vitro blood brain barrier (BBB) models, interaction between BBB and GBM was explored. In acute phase of the interaction, GBM could strengthen BBB, and FGF-2 might be implicated in the process. These results have been published.

Research Products

• [Journal Article] Initial Contact of Glioblastoma Cells with Existing Normal Brain Endothelial Cells Strengthen the Barrier Function via Fibroblast Growth Factor 2 Secretion : A New In Vitro Blood-Brain Barrier Model (2013)
  • Author(s): Toyoda K, Tanaka K, Nakagawa S, Thuy DH, Ujifuku K, Kamada K, Hayashi K, Matsuo T, Nagata I, Niwa M
  • Journal Title: Cell Mol Neurobiol Volume: 33(4) Pages: 489-501
  • DOI: 10.1007/s10571-013-9913-z.
  • Peer Reviewed
• [Journal Article] Repeated delayed onset cerebellar radiation injuries after linear accelerator-based stereotactic radiosurgery for vestibular schwannoma : case report (2012)
  • Author(s): Ujifuku K, Matsuo T, Toyoda K, Baba S, Okunaga T, Hayashi Y, Kamada K, Morikawa M, Suyama K, Nagata I, Hayashi N
  • Journal Title: Neurol Med Chir (Tokyo) Volume: 52(12) Pages: 933-936
  • URL: https://www.jstage.jst.go.jp/article/nmc/52/12/52_2947/_article
  • Peer Reviewed
• [Journal Article] 長崎大学病院救命救急センター設立前後における脳神経外科救急の動向 (2012)
  • Author(s): 氏福健太、福田修志、豊田啓介、畑地豪、中尾健次郎、中嶋秀樹、立石洋平、馬場史郎、猪熊孝実、浅原智彦、山下和範、林健太郎、余縄雅彦、陶山一彦、長谷敦子、澄川耕二、永田泉
  • Journal Title: Neurosurg Emerg Volume: 17 Pages: 8-14
  • Peer Reviewed
• [Journal Article] miR-196a Downregulation Increases the Expression of Type I and III Collagens in Keloid Fibroblasts (2012)
  • Author(s): Kashiyama K, Mitsutake N, Matsuse M, Ogi T, Saenko VA, Ujifuku K, Utani A, Hirano A, Yamashita S
  • Journal Title: J Invest Dermatol Volume: 132(6) Pages: 1597-604
  • DOI: 10.1038/jid.2012.22.
  • Peer Reviewed
• [Journal Article] MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells (2011)
  • Author(s): Matsushima K, Isomoto H, Yamaguchi N, Inoue N, Ujifuku K, Mitsutake N, Machida H, Nakayama T
  • Journal Title: J Transl Med Volume: 9 Pages: 30
  • DOI: 10.1186/1479-5876-9-30
  • Peer Reviewed
• [Journal Article] A case of multinodular high-grade neuroepithelial tumor with ependymal differentiation (2011)
  • Author(s): Kamada K, Tanaka Y, Matsuo T, Baba S, Ujifuku K, Suyama K, Nakazato Y, Nagata I
  • Journal Title: Brain Tumor Pathol Volume: 28(3) Pages: 253-7
  • DOI: 10.1007/s10014-011-0032-6
  • Peer Reviewed
• [Presentation] 脳腫瘍におけるfetal cell microchimerism (2012)
  • Author(s): 出端亜由美、馬場史郎、氏福健太、吉田光一、鎌田健作、松尾孝之、永田泉、林徳眞吉
  • Organizer: 第30回日本脳腫瘍学会学術集会
  • Place of Presentation: グランドプリンスホテル広島
  • Year and Date: 2012-11-26
• [Presentation] Aggressive intracranial fibromatosisの一剖検例 (2012)
  • Author(s): 氏福健太、鎌田健作、定方英作、出端亜由美、馬場史郎、吉田光一、松尾孝之、森川実、下川功、安部邦子、中里洋一、永田泉
  • Organizer: 第30回日本脳腫瘍学会学術集会
  • Place of Presentation: グランドプリンスホテル広島
  • Year and Date: 2012-11-25
• [Presentation] 最近7年間における慢性硬膜下血腫治療の現状–長崎大学病院の場合 (2012)
  • Author(s): 氏福健太、定方英作、前田肇、山口将、馬場史郎、吉田光一、堀江信貴、出雲剛鎌、田健作、角田圭司、林健太郎、松尾孝之、森川実、永田泉
  • Organizer: 日本脳神経外科学会第71回学術総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2012-10-17
• [Presentation] 脳神経外科悪性腫瘍臨床における緩和ケアの役割～長崎大学病院の場合 (2011)
  • Author(s): 氏福健太、高田正史、出端亜由美、豊田啓介、馬場史郎、吉田光一、鎌田健作、松尾孝之、陶山一彦、永田泉、澄川耕二、北條美能留
  • Organizer: 第29回日本脳腫瘍学会学術集会
  • Place of Presentation: 下呂温泉水明館
  • Year and Date: 2011-11-27
• [Presentation] Palliative care for patients with malignant tumor in the Department of Neurosurgery (2011)
  • Author(s): Kenta Ujifuku, Masafumi Takada, Ayumi Debata, Keisuke Toyoda, Shiro Baba, Koichi Yoshida, Kensaku Kamada, Takayuki Matsuo, Kazuhiko Suyama, Izumi Nagata, Koji Sumikawa and Minoru Hojo
  • Organizer: The 12th Young-Honam and Kyushu Neurosurgical Joint Meeting in Kitakyushu
  • Place of Presentation: リーガロイヤルホテル小倉
  • Year and Date: 2011-11-12
• [Presentation] 5-aminolevulinic acidを用いた術中蛍光診断の感度と特異度長崎大学病院において原発性脳腫瘍をターゲットとした場合 (2011)
  • Author(s): 氏福健太、林之茂、山口将、豊田啓介、馬場史郎、吉田光一、鎌田健作、森川実、林徳眞吉、陶山一彦、永田泉、松尾孝之
  • Organizer: (社)日本脳神経外科学会第70回学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-10-13
• [Presentation] 長崎大学病院救命救急センター設立前後における脳神経外科救急の動向 (2011)
  • Author(s): 氏福健太、馬場史郎、畑地豪、中尾健次郎、中嶋秀樹、立石洋平、猪熊孝実、浅原智彦、林健太郎、山下和範、余縄雅彦、陶山一彦、長谷敦子、澄川耕二、永田泉
  • Organizer: 第15回日本救急医学会九州地方会
  • Place of Presentation: アルカス佐世保
  • Year and Date: 2011-05-28
• [Remarks] 長崎大学脳神経外科ホームページ
  • URL: http://www.med.nagasaki-u.ac.jp/nsurgery/link/index.htm