Study of the molecular mechanisms of GlcN for antiinflammatory action in synovial cells.

2014 Fiscal Year Final Research Report

• Project/Area Number: 23592232
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Juntendo University
• Principal Investigator: SOMEYA AKIMASA 順天堂大学, 医学部, 准教授 (90167479)
• Co-Investigator(Kenkyū-buntansha): NAGAOKA Isao 順天堂大学, 医学部, 教授 (60164399)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: グルコサミン / 抗炎症 / 滑膜細胞 / O-GlcNAc修飾 / 機能性食品

Outline of Final Research Achievements

Glucosamine (GlcN) is widely used as a dietary supplement to treat osteoarthritis (OA) in humans. An anti-inflammatory action of GlcN is speculated to be involved in its protective actions on OA. However, its precise mechanism is not clear. In this research project, we examined the molecular mechanisms of GlcN for anti-inflammatory action in synovial cells. GlcN induced the O-N-acetylglucosamine (O-GlcNAc) modification of cellular proteins, and transcription factor Sp1 was identified as O-GlcNAc modified protein by GlcN. Sp1 was involved in the regulation of IL-8 production. Furthermore, GlcN moderated transcription activity of Sp1 and intranuclear amount of Sp1 via the O-GlcNAc modification. Together these observations suggest that GlcN alleviates synovial inflammation by the suppression of proinflammatory cytokines, such as IL-8, via O-GlcNAc modification of Sp1.

Free Research Field

生化学