Development of a novel one-stepimplantation technique for articular cartilage repair using human minced cartilage and isolated synovial cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592235
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Toho University
• Principal Investigator: NAKAGAWA Koichi 東邦大学, 医学部, 教授 (30400823)
• Co-Investigator(Kenkyū-buntansha): AOKI Yasuchika 東邦大学, 医学部, 准教授 (70584001) ; NAKAJIMA Arata 東邦大学, 医学部, 准教授 (60583995)
• Co-Investigator(Renkei-kenkyūsha): SASHO Takahisa 千葉大学, 大学院医学研究院, 准教授 (20312952)
• Project Period (FY): 2011 – 2013
• Keywords: 関節病学 / 軟骨再生

Research Abstract

[Objective] To examine the effect of co-culture or co-implantation of synovial and minced cartilage on the chondrogenic differentiation. [Methods] Human synovial cells were isolated and embedded in fibrin gel. The fibrin gel implants containing only synovial cells (SC group) or both minced articular cartilage and synovial cells (AC+SC group) were cultured or implanted subcutaneously in nude mice and then examined the histological and biochemically. [Results] Intensity of Safranin-O staining and proteoglycan content (per weight) increased in the AC+SC group compared to the SC group. Furthermore, type II collagen and Sox9 expression were enhanced in the AC+SC group. [Conclusion] Co-culture or co-implantation of synovial cells with minced cartilage enhanced chondrogenic differentiation. The use of implant grafts containing both synovial cells and minced cartilage could be a novel approach for one-step joint cartilage repair.

Research Products

• [Journal Article] Changes in synovial fluid biochemical markers following arthroscopic surgery in patients with knee osteoarthritis (2013)
  • Author(s): Nakajima A, Nakagawa K, Aoki Y, Sonobe M, Shibata Y, Yamazaki M, Murakami M
  • Journal Title: Rheumatol Int Volume: 33(1) Pages: 209-214
  • Peer Reviewed
• [Journal Article] Failure of conservative treatment for thoracic spine fracture in ankylosing spondylitis : delayed neurological deficit due to spinal epidural hematoma (2013)
  • Author(s): Aoki Y, Yamagata M, Ikeda Y, Nakajima F, Nakajima A, Nakagawa K, Ohtori S, Inaoka T, Takahashi K
  • Journal Title: Mod Rheumatol Volume: 23(5) Pages: 1008-1012
  • Peer Reviewed
• [Journal Article] Clinical value of routine use of thin-section 3D MRI using 3D FSE sequences with a variable flip angle technique for internal derangements of the knee joint at 3T (2013)
  • Author(s): Kudo H, Inaoka T, Kitamura N, Nakatsuka T, Kasuya S, Kasai R, Tozawa M, Nakagawa K, Terada H
  • Journal Title: Magn Reson Imaging Volume: 31(8) Pages: 1309-1317
  • Peer Reviewed
• [Journal Article] 近位および遠位リアライメントを同時に施行した膝蓋骨脱臼症例の経験 (2013)
  • Author(s): 園部正人、中川晃一、谷口慎治、青木保親、中島新、寺島史明、齊藤雅彦、高橋宏、山田学、北原聡太、池川直志
  • Journal Title: 千葉スポーツ医学研究会雑誌 Volume: 10 Pages: 13-17
  • Peer Reviewed
• [Journal Article] 関節リウマチ患者の荷重関節に対するエタネルセプトの関節破壊抑制効果 (2013)
  • Author(s): 園部正人、中島新、窪田綾子、中川晃一、勝呂徹
  • Journal Title: 関節の外科 Volume: 40 Pages: 82-86
  • Peer Reviewed
• [Journal Article] The use of three visual analogue scales to evaluate back pain of patients motion, standing and sitting (2012)
  • Author(s): Aoki Y, Nakagawa K, Nakajima A, Shibata Y, Sonobe M, Ohtori S, Furufu T, Takahashi K
  • Journal Title: J Spine Res Volume: 3 Pages: 863-866
  • Peer Reviewed
• [Journal Article] Angiogenic activity of subchondral bone during the progression of osteoarthritis in a rabbit anterior cruciate ligament transection model (2012)
  • Author(s): Saito M, Sasho T, Yamaguchi S, Ikegawa N, Akagi R, Muramatsu Y, Mukoyama S, Ochiai N, Nakamura J, Nakagawa K, Nakajima A, Takahashi K
  • Journal Title: Osteoarthritis Cartilage Volume: 20(12) Pages: 1574-1582
  • Peer Reviewed
• [Journal Article] Maturation-dependent spontaneous healing of partial thickness cartilage defects in infantile rats (2011)
  • Author(s): Tsuruoka H, Sasho T, Yamaguchi S, Ikegawa N, Saito M, Akagi R, Ochiai N, Nakagawa K, Nakajima A, Fallouh L, Takahashi K
  • Journal Title: Cell Tissue Res Volume: 346(2) Pages: 261-271
  • Peer Reviewed
• [Presentation] 広範囲関節軟骨欠損に対する骨髄刺激とフィブロイン被覆の併用療法の効果 (2013)
  • Author(s): 中川晃一、富田直秀、玉田靖、中島新、柴田孝史、園部正人、北原聡太、青木保親、高橋宏、齋藤雅彦、谷口慎治、山田学、平方栄一、青木秀之、齋藤知行
  • Organizer: 第41回日本関節病学会(OAの病態を考慮した治療の基礎と臨床)
  • Place of Presentation: 名古屋 (パネルディスカッション)
  • Year and Date: 20131102-03
• [Presentation] イヌ広範囲関節軟骨欠損の修復過程におけるフィブロイン被覆法の初期効果 (2013)
  • Author(s): 中川晃一, 富田直秀, 玉田靖, 柴田孝史, 園部正人, 中島新, 青木保親, 平方栄一, 青木秀之, 齋藤知行
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉
  • Year and Date: 20131017-18
• [Presentation] 関節リウマチ患者における血中酸化ストレスの臨床的意義と滑膜線維芽細胞における活性酸素種(ROS)産生 (2013)
  • Author(s): 中島新, 青木保親, 園部正人, 寺島史明, 高橋宏, 齊藤雅彦, 谷口慎治, 山田学, 後藤憲一郎, 田原正道, 山中一, 玉井浩, 小林達也, 中川晃一
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉
  • Year and Date: 20131017-18
• [Presentation] 関節リウマチ滑膜組織におけるIL-17、IFN-γ、TNF-αの発現と関節破壊制御のメカニズム (2013)
  • Author(s): 中島新, 青木保親, 園部正人, 寺島史明, 高橋宏, 齊藤雅彦, 谷口慎治, 山田学, 後藤憲一郎, 田原正道, 山中一, 玉井浩, 小林達也, 中川晃一
  • Organizer: 第28回日本整形外科学会基礎学術集会
  • Place of Presentation: 千葉
  • Year and Date: 20131017-18
• [Presentation] (軟骨再生医療の最前線)フィブロインのcell delivery機能を利用した関節軟骨再生法の開発 (2013)
  • Author(s): 中川晃一、富田直秀、玉田靖、平方栄一、青木秀之、園部正人、柴田孝史、中島新、齋藤知行
  • Organizer: 第5回日本関節鏡・膝・スポーツ整形外科学会(JOSKAS)
  • Place of Presentation: 札幌 (シンポジウム)
  • Year and Date: 20130620-22
• [Presentation] Should the presence of back pain be considered when making surgical decisions for lumbar spinal fusion? (2013)
  • Author(s): Aoki Y, Nakagawa K, Nakajima A, Shibata Y, Sonobe M, Takahashi H, Taniguchi S, Yamada M
  • Organizer: Should the presence of back pain be considered when making surgical decisions for lumbar spinal fusion? International Society for Minimally Intervention in Spinal Surgery Japan
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20130620-21
• [Presentation] (主題;生物学的製剤使用下でのリウマチ手術)生物学的製剤使用・非使用下における術後創傷遅延、感染症発生の検討 (2013)
  • Author(s): 中島新, 園部正人, 谷口慎治 ,中川晃一
  • Organizer: 第42回リウマチの外科研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2013-08-31
• [Presentation] (基礎研究をもとにした軟骨修復および再生への新たな工夫)関節軟骨修復術とMRIによる組織評価 (2012)
  • Author(s): 山下剛司、渡辺淳也、本田英義、佐粧孝久、中川晃一、豊根知明、和田佑一
  • Organizer: 第27回日本整形外科学会基礎学術集会
  • Place of Presentation: 名古屋 (シンポジウム)
  • Year and Date: 20121026-27
• [Presentation] Improvement of back pain following lumbar decompression surgery : Evaluation by visual analogue scale for patients in motion, standing and sitting (2012)
  • Author(s): Aoki Y, Nakagawa K, Nakajima A, Shibata Y, Sonobe M, Ohtori S, Takahashi K
  • Organizer: 39th International Society for the study of the Lumbar Spine (ISSLS)
  • Place of Presentation: Amsterdam, Netherland
  • Year and Date: 20120528-0601
• [Presentation] Discogenic Painの基礎(Modicを含む) (2012)
  • Author(s): 青木保親、中川晃一、中島新、大鳥精司、高橋和久
  • Organizer: 第38回日本整形外科スポーツ医学会学術集会
  • Place of Presentation: 横浜 (パネルディスカッション)
  • Year and Date: 2012-09-14
• [Presentation] Co-implantation with minced articular cartilage enhances chondrogenic differentiation of synovial cells (2011)
  • Author(s): Fallouh L, Moriya H, Takahashi K, Nakagawa K, Sasho T, Kitahara S, Moriya T, Ikegawa N, Saito M, Akagi R, Wada Y
  • Organizer: The 8^<th> Biennial Congress of International Society of Arthroscopy, Knee Surgery & Orthopaedic Sports Medicine (ISAKOS)
  • Place of Presentation: Rio De Janeiro, Brazil
  • Year and Date: 20110515-19
• [Patent(Industrial Property Rights)] 滑膜細胞および細切軟骨辺の軟骨修復における使用 (2013)
  • Inventor(s): 中川晃一
  • Industrial Property Rights Holder: 国立大学法人 千葉大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特許第5373427号
  • Filing Date: 2013-09-27
  • Overseas