The neuroprotective effects of anesthetics against hypoxia

2014 Fiscal Year Final Research Report

• Project/Area Number: 23592253
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Osaka University
• Principal Investigator: SHIBUTA Satoshi 大阪大学, 医学(系)研究科(研究院), 助教 (20324767)
• Co-Investigator(Kenkyū-buntansha): KAMIBAYASHI Takahiko 大阪大学, 大学院医学系研究科, 講師 (10273640) ; MIYAMOTO Yoshikazu 独立行政法人大阪府立病院機構, 大阪府立母子保健総合医療センター, 医員 (70278844) ; KOSAKA Jun 国際医療福祉大学, 保健医療学部, 教授 (40243216)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 脳神経 / 麻酔薬

Outline of Final Research Achievements

The neurotoxic effects of anesthetics on the developing brain are a concern.
We examined the degree of L-glutamate-induced intracellular calcium ([Ca2+]i) elevation and neurotoxicity in primary cultured neurons exposed to anesthetics.The primary cortical neurons from E17 Wistar rats were preincubated with various concentrations of ketamine or thiopental sodium (TPS) for the first 72 h of culturing. Two weeks later, the neurons were exposed to L-glutamate. The extent of glutamate toxicity was evaluated by Ca-imaging and morphological experiments.Although preincubation with extra high concentration of ketamine showed enhancement of L-glutamate-induced [Ca2+]i elevation 2 weeks later, long-term exposure to TPS or ketamine at clinical dose during developmental periods may not result in a dose-related potentiation of exogenous glutamate-induced neurotoxicity, once the intravenous anesthetics are removed.

Free Research Field

麻酔学