The pharmacological analysis of anesthetics in Dorsal root ganglia

2014 Fiscal Year Final Research Report

• Project/Area Number: 23592263
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Jichi Medical University
• Principal Investigator: OGATA Junichi 自治医科大学, 医学部, 講師 (50352331)
• Co-Investigator(Kenkyū-buntansha): 南 浩一郎 自治医科大学, 医学部, 講師 (70279347) ; 横山 徹 自治医科大学, 医学部, 助教 (80425321) ; 上園 保仁 独立行政法人国立がん研究センター, がん患者病態生理研究分野, 分野長 (20213340)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 脊髄後根神経節細胞 / G蛋白共役型受容体 / カルシウム動態 / アフリカツメガエル卵母細胞発現系 / イオンチャネル / トラマドール / TRIPV1 / TRIPA1

Outline of Final Research Achievements

G-protein-coupled receptors, such as opioid receptors, substance P receptors (Sub P receptor) have been attracting attention for pain mechanisms. On the other hand, the spinal cord dorsal root ganglion (DRG) cells are involved to pain, such as Sub-P receptor and opioid receptors are present. In this study, we studied the effects of anesthetics and analgesics on TRIPV1, opioid receptors and Sub P receptors using calcium kinetics in DRG and the Xenopus oocyte expression system. Consequently, inhaled anesthetics sevoflurane inhibited could opioid receptor function. Furthermore, analgesic tramadol and its metabolite M1 is no effect on TRIPV1, was demonstrated to inhibit TRIPA1.

Free Research Field

麻酔学