Functional analysis of isolated specific antibody to prostate cancer specific antigen using phage-display method

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592353
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Fujita Health University
• Principal Investigator: SHIROKI Ryoichi 藤田保健衛生大学, 医学部, 教授 (70226330)
• Co-Investigator(Kenkyū-buntansha): HOSHINAGA Kiyotaka 藤田保健衛生大学, 医学部, 教授 (30229174) ; AKAHORI Yasushi 三重大学, 医学系研究科, 産学官連携講座講師 (80221711)
• Project Period (FY): 2011 – 2013
• Keywords: 前立腺癌 / 前立腺膜特異的抗原（PSMA) / 癌特異的抗原 / 抗体

Research Abstract

In Antibody project in Comprehensive Research Institute of Fujita Heath University, Human phage antibody library; AIMS5 library, was established using human extracted B lymphocyte from the almond of dozens of people, marrow, peripheral blood, umbilical cord blood. Applying the phage-display method, specific antibody to PSMA, prostate specific membrane antibody, (75-10) was isolated from this library using ICOS method, J Immunol Methods. 351:1-12, 2009. Specific antibody to PSMA (75-10) was demonstrated to show the specific prostate membane-specific reactivity to prostate cancer tissue (the specific stainability to cell surface membrane) and an in vitro cancer cytotoxicity in cultured and a clinical specimen of the prostate cancer.

Research Products

• [Journal Article] Serum neutrophil gelatinase associated lipocalin during the early postoperative period predicts the recovery of graft function after kidney transplantation from donors after cardiac death (2012)
  • Author(s): Kusaka M,Iwamatsu F,Kuriyanagi Y,Nakaya M,Ichino M,Marubashi S,Nagano H,Shiroki R,Kurahashi H,Hoshinaga K
  • Journal Title: J Urol Volume: 187 Pages: 2261-2267
  • Peer Reviewed
• [Journal Article] ロボット支援前立腺全摘除術の導入と手術手技―リンパ節郭清を中心に (2012)
  • Author(s): 白木良一、丸山高広、石瀬仁司、日下守、森川高光、深谷孝介、深見直彦、佐々木ひと美、石川清仁、星長清隆
  • Journal Title: 日本泌尿器内視鏡学会雑誌 Volume: 25巻2号 Pages: 230-236
  • Peer Reviewed
• [Journal Article] Selection and analysis of anti-cancer antibodies for cancer therapy obtained from antibody phage library (2011)
  • Author(s): Kurosawa G,Sumitomo M,Ukai Y,Subera J,Muramatsu C,Eguchi K,Tanaka-Hashiba M,Sugiura M,Ando M,Morita M,Inaba K,Morigaki S,Takasaki A,Miyakawa S,Uyama I,Maeda K,Shiroki R,Hoshinaga K,Mizoguchi Y,Hattori Y,Sugioka A,Sugiura M,Kurosawa Y
  • Journal Title: Cancer Science Volume: 102 Pages: 175-181
  • Peer Reviewed
• [Journal Article] Transurethral resection in saline vaporization : Evaluation of clinical efficacy and prostate volume (2011)
  • Author(s): Otsuki H,Kuwahara Y,Kosaka T,Tsukamoto T,Nakamura K,Shiroki R,Hoshinaga K
  • Journal Title: Urology Volume: Vol.79,No.3 Pages: 665-669
  • Peer Reviewed
• [Journal Article] Photoselective vaporization of the prostate : pursuing good indications based on the results of 400 Japanese patients (2011)
  • Author(s): Otsuki H,Kuwahara Y,Tsukamoto T,Nakamura K,Shiroki R,Hoshinaga K
  • Journal Title: BJU International Volume Volume: Vol.109,No.6 Pages: 874-879
  • Peer Reviewed
• [Journal Article] ホルミウムレーザー前立腺核出術(HoLEP)の周術期抗菌薬予防投与の現状とアンケート調査の結果について (2011)
  • Author(s): 石川清仁、丸山高広、日下守、白木良一、星長清隆
  • Journal Title: 泌尿器科紀要 Volume: 57巻10号 Pages: 539-543
  • Peer Reviewed
• [Presentation] Joint session of the European association of urology(EAU)and the Japanese urological association robot assisted radical prostatectomy (2013)
  • Author(s): Shiroki R
  • Organizer: 28th Annual EAU Congress
  • Place of Presentation: Milan, Italy
  • Year and Date: 20130000
• [Presentation] Isolation of anti PSMA antibody and screening for antigens overexpressed on prostate cancer using anticancer antibodies obtained from antibody phage-display library (2013)
  • Author(s): Hikichi M,Shiroki R,Kurosawa G,Akahori Y,Hoshinaga K,Kurosawa Y
  • Organizer: 33rd Congress of the Societe International Urology
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 20130000
• [Presentation] Robot -assisted laparoscopic partial Nephrectomy for T1 renal tumor initial series at Fujita Health University (2013)
  • Author(s): Ishise H,Shiroki R,Fukaya K,Fukami N,Kusaka M,Hoshinaga K
  • Organizer: 30th Anniversary Korea-Japan Urological Congress
  • Place of Presentation: Seoul, Korea
  • Year and Date: 20130000
• [Presentation] Nerve-sparing procedure impacts on early recovery of postopetative urinary continence in patients treated with total urinary reconstruction technique in Robot-Assisted Radical Prostatectomy (2012)
  • Author(s): Shiroki R,Maruyama T,Kusaka M, Hoshinaga K
  • Organizer: 32nd Congress of the Societe International Urologie
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 20120000
• [Presentation] 抗PSMA抗体と様々な癌特異抗原に対する抗体を用いた前立腺臨床検体における発現解析 (2012)
  • Author(s): 引地克、白木良一、赤堀泰、黒澤仁、黒澤良和、星長清隆
  • Organizer: 第100回日本泌尿器科学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20120000
• [Presentation] 当院における去勢抵抗性前立腺癌(CRPC)に対するDoxetaxel療法の長期成績 (2011)
  • Author(s): 引地克,白木良一,河合昭浩,西野将,深谷孝介,彦坂和信,平野泰広,深見直彦,石瀬仁司,丸山高広,佐々木ひと美,日下守,石川清仁,星長清隆
  • Organizer: 第49回日本癌治療学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 20110000
• [Presentation] 抗PSMA抗体を用いた前立腺癌臨床検体の発現プロファイル解析 (2011)
  • Author(s): 引地克、白木良一、西野将、河合昭浩、深谷孝介、彦坂和信、平野泰広、石瀬仁司、深見直彦、丸山高広、佐々木ひと美、日下守、石川清仁、星長清隆
  • Organizer: 第61回日本泌尿器科学会中部総会
  • Place of Presentation: 京都
  • Year and Date: 20110000