2013 Fiscal Year Final Research Report
Analysis of spermatogenesis with reduced quality of the genome in degenerate testis of DNA repair-deficient mice
| Project/Area Number |
23592379
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | DNA修復欠損マウス / 精子形成 / 精巣変性状態 / ゲノム不安定性 |
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| Research Abstract |
We have showed that xeroderma pigmentosum group A (Xpa) gene-knockout mice [Xpa (-/-) mice] provide a valuable model for impaired spermatogenesis as well as for UV-induced skin tumors in XPA patients. Gene expression analysis of each aged Xpa mice testis showed a different expression pattern of DNA repair gene, cell cycle-related gene(checkpoint), apoptosis-related gene compared to control. We tried to check the different expression pattern of these genes by Real-time PCR and immunohistochemistry. We did not detected these difference of these genes by the above methods. However, we found other pathology of vacuole formation in the aged Xpa mice testis. These findings will help us to understand the impaired spermatogenesis in the Xpa mice.
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[Presentation] Histopathological analysis of kidney in Xpg null mice2014
Author(s)
H Nakane , T Shiomi , T Kameie, S Inaga, T Naguro , T Kaidoh
Organizer
The International Symposium on Xeroderma Pigmentosum and Related Diseases : Disorders of DNA Damage Response -Bench to Bedside-
Place of Presentation
Kobe International Conference Center (Kobe, Hyogo)
Year and Date
20140305-07
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[Presentation] Immunohistochemical evaluation of impaired spermatogenesis in xeroderma pigmentosum group A gene (Xpa)-deficient mice2013
Author(s)
Nakane, H., Tanaka, K., Kameie, T., Inaga, S., Naguro, T., Kaidoh, T.
Organizer
第36回日本分子生物学会年会
Place of Presentation
神戸国際展示場(兵庫県神戸市)
Year and Date
20131203-06
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