2013 Fiscal Year Final Research Report
Development of the method of selecting the sperm in the ART (Assisted reproductive technology)
| Project/Area Number |
23592420
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
SUGIYAMA Hitoshi 沖縄科学技術大学院大学, サイエンス・テクノロジー・グループ, リサーチサイエンティスト (70301596)
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| Co-Investigator(Kenkyū-buntansha) |
FUNABASHI Toshiya 聖マリアンナ医科大学, 医学部, 教授 (70229102)
YAGI Chiaki 琉球大学, 医学部付属病院, 助教 (20529341)
ASATO Kozue 琉球大学, 医学部付属病院, 助教 (30452246)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 生殖医療 / 精子 / 走化性 / 受精 |
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| Research Abstract |
In in vitro fertilization using intracytoplasmic sperm injection (ICSI), a single sperm is injected directly into an egg. Although the physiological separation acts in the natural pregnancy, a sperm provided to ICSI is chosen by an embryologist. We customized method of ICSI and some methods for analyses of the sperm DNA fragmentation in mice to perform in our laboratory, and then it was examined whether chemotaxis could use for mammalian sperm separation in this study, but it could not detect the chemotaxis by methods that we used.
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