The study aiming at non-invasive prenatal diagnosis: establishment of fetal cell separation from peripheral blood of pregnant women during the first trimester.

2014 Fiscal Year Final Research Report

• Project/Area Number: 23592424
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Aichi Medical University
• Principal Investigator: SONTA Shinichi 愛知医科大学, 公私立大学の部局等, 客員研究員 (00100165)
• Co-Investigator(Kenkyū-buntansha): KUROKAWA Kei 日本赤十字豊田看護大学, 看護学部, 教授 (90399030) ; SAGA Shinsuke 愛知医科大学, 医学部, 教授 (40144141) ; WAKATSUKI Akihiko 愛知医科大学, 医学部, 教授 (90191717)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 染色体異常 / トリソミー / 出生前診断 / 非侵襲性検査

Outline of Final Research Achievements

Due to try to sort out fetal cells from maternal blood in the pregnant woman, the density gradient centrifugation was repeated. The fluid with rich fetal cells was put in the culture chamber which applied the fetal cell-specific antibody. The fetal cells were adherent partially by this method. In addition to the known antibody, we couldn't get useful information about nano antibodies in particular in fetal cells. The dissociation procedures of fetal nRBCs by FACS was also considered. The slide preparations were made with cells concentrated by these methods. Fetal nRBCs were identified by the morphological specificity. The chromosome construction of these fetal cells was confirmed by FISH using probes which is able to distinguish each chromosome. Completion of the study may enable "prenatal diagnosis " of fetal chromosomes.

Free Research Field

臨床細胞遺伝学