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2013 Fiscal Year Final Research Report

Identification of stem cell marker of mouse corneal epithelial cell

Research Project

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Project/Area Number 23592607
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ophthalmology
Research InstitutionEhime University

Principal Investigator

SHIRAISHI Atsushi  愛媛大学, 医学(系)研究科(研究院), 准教授 (90314963)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Takeshi  愛媛大学, 大学院医学系研究科, 寄附講座助教 (70380285)
SHIRAKATA Yuji  愛媛大学, 大学院医学系研究科, 准教授 (50226320)
Project Period (FY) 2011 – 2013
Keywords再生医療 / 角膜上皮 / 形質転換
Research Abstract

We have established the mouse model for trans-differentiation from epidermal cell to corneal epithelial cell using K12-Cre-Zeg mouse in which green fluorescein protein (GFP) is expressed driven by Keratin 12 promoter. We first examined immunohistochemical staining, and results showed that K 10 was expressed in the center of the cornea and K10 and K12 were expressed in the limbus of the cornea in the trans-differentiation model. When side population cells (Sp cells) isolated from epidermis were co-cultured with cornel keratocyte isolated from corneal limbus, GFP positive cells were detected. RT-PCR results also showed positive for K 12. We then employed transcriptome analysis between the keratocytes isolated from central and limabal cornea. The results showed high expression of Wnt signal related mRNA were detected.

  • Research Products

    (22 results)

All 2014 2013 2012 2011

All Journal Article (14 results) Presentation (8 results)

  • [Journal Article] Evaluation of allergic conjunctivitis by thermography2014

    • Author(s)
      Hara Y, Shiraishi A, Yamaguchi M, Kawasaki S, Uno T, Ohashi Y
    • Journal Title

      Ophthalmic Res

      Volume: 51(3) Pages: 161-6

  • [Journal Article] Coincidence of Varicella-Zoster Virus Anterior Uveitis in a Patient with Chandler's Syndrome2013

    • Author(s)
      Joko T, Suzuki T, Inoue T, Kikuchi M, Hara Y, Shiraishi A, Ohashi Y
    • Journal Title

      Case Rep Ophthalmol

      Volume: 4(3) Pages: 274-8

  • [Journal Article] New side-view imaging technique for observing posterior chamber structures during cataract surgery in porcine eyes2013

    • Author(s)
      Tasaka Y, Minami N, Suzuki T, Kawasaki S, Zheng X, Shiraishi A, Uno T, Miyake K, Ohashi Y
    • Journal Title

      BMC Ophthalmol

      Volume: 13 Pages: 47

  • [Journal Article] In vitro efficacy of ocular surface lubricants against dehydration2013

    • Author(s)
      Zheng X, Goto T, Shiraishi A, Ohashi Y
    • Journal Title

      Cornea

      Volume: 32(9) Pages: 1260-4

  • [Journal Article] Involvement of P38MAPK in human corneal endothelial cell migration induced by TGF-β(2)2013

    • Author(s)
      Joko T, Shiraishi A, Akune Y, Tokumaru S, Kobayashi T, Miyata K, Ohashi Y
    • Journal Title

      Exp Eye Res

      Volume: 108 Pages: 23-32

  • [Journal Article] Effect of photodynamic therapy with methylene blue on Acanthamoeba in vitro2012

    • Author(s)
      Mito T, Suzuki T, Kobayashi T, Zheng X, Hayashi Y, Shiraishi A, Ohashi Y
    • Journal Title

      Invest Ophthalmol Vis Sci

      Volume: 53(10) Pages: 6305-13

  • [Journal Article] Blepharo-tensiometer : new eyelid pressure measurement system using tactile pressure sensor2012

    • Author(s)
      Sakai E, Shiraishi A, Yamaguchi M, Ohta K, Ohashi Y
    • Journal Title

      Eye Contact Lens

      Volume: 38(5) Pages: 326-30

  • [Journal Article] Association of Toll-like receptor 4 gene polymorphisms in Japanese subjects with primary open-angle, normal-tension, and exfoliation glaucoma2012

    • Author(s)
      Takano Y, Shi D, Shimizu A, Funayama T, Mashima Y, Yasuda N, Fukuchi T, Abe H, Ideta H, Zheng X, Shiraishi A, Ohashi Y, Nishida K, Nakazawa T, Fuse N
    • Journal Title

      Am J Ophthalmol

      Volume: 154(5) Pages: 825-832.e1

  • [Journal Article] Involvement of stem cell factor and c-kit in corneal wound healing in mice2012

    • Author(s)
      Miyamoto K, Kobayashi T, Hayashi Y, Zhang Y, Hara Y, Higashine M, Shiraishi A, Ohashi Y
    • Journal Title

      Mol Vis

      Volume: 18 Pages: 1505-15

  • [Journal Article] Important role of epiregulin in inflammatory responses during corneal epithelial wound healing2012

    • Author(s)
      Zhang Y, Kobayashi T, Hayashi Y, Yoshioka R, Shiraishi A, Shirasawa S, Higashiyama S, Ohashi Y
    • Journal Title

      Invest Ophthalmol Vis Sci

      Volume: 53(4) Pages: 2414-23

  • [Journal Article] Assessment of real-time polymerase chain reaction detection of Acanthamoeba and prognosis determinants of Acanthamoeba keratitis2012

    • Author(s)
      Ikeda Y, Miyazaki D, Yakura K, Kawaguchi A, Ishikura R, Inoue Y, Mito T, Shiraishi A, Ohashi Y, Higaki S, Itahashi M, Fukuda M, Shimomura Y, Yagita K
    • Journal Title

      Ophthalmology

      Volume: 119(6) Pages: 1111-9

  • [Journal Article] Use of 5-cyano-2,3-ditolyl-tetrazolium chloride staining as an indicator of biocidal activity in a rapid assay for anti-Acanthamoeba agents2012

    • Author(s)
      Kobayashi T, Mito T, Watanabe N, Suzuki T, Shiraishi A, Ohashi Y
    • Journal Title

      J Clin Microbiol

      Volume: 50(5) Pages: 1606-12

  • [Journal Article] Confocal microscopic observations of stromal keratocytes in soft and rigid contact lens wearers2012

    • Author(s)
      Ohta K, Shimamura I, Shiraishi A, Ohashi Y
    • Journal Title

      Cornea

      Volume: 31(1) Pages: 66-73

  • [Journal Article] Mathematical projection model of visual loss due to fuchs corneal dystrophy2011

    • Author(s)
      Hatou S, Shimmura S, Shimazaki J, Usui T, Amano S, Yokogawa H, Kobayashi A, Zheng X, Shiraishi A, Ohashi Y, Inatomi T, Tsubota K
    • Journal Title

      Invest Ophthalmol Vis Sci

      Volume: 52(11) Pages: 7888-93

  • [Presentation] 渦状角膜症における生体共焦点顕微鏡所見の検討2013

    • Author(s)
      池川泰民, 白石敦, 大木元明義, 大橋裕一
    • Organizer
      第67回日本臨床眼科学会
    • Place of Presentation
      横浜
    • Year and Date
      20131031-1103
  • [Presentation] エアオプテイクスEXアクア治療的使用の実際2013

    • Author(s)
      井上智之, 鈴木崇, 原祐子, 鄭暁東, 林康人, 山口昌彦, 白石敦, 大橋裕一
    • Organizer
      フォーサム2013
    • Place of Presentation
      大阪
    • Year and Date
      20130712-14
  • [Presentation] 高張食塩水を用いたポートレス・ドレナージDSAEKの術後成績2012

    • Author(s)
      原祐子, 鳥山浩二, 坂根由梨, 鈴木崇, 鄭暁東, 宇野敏彦, 白石敦, 大橋裕一
    • Organizer
      第66回日本臨床眼科学会
    • Place of Presentation
      京都
    • Year and Date
      20121025-28
  • [Presentation] 水疱性角膜症に対する角膜移植の多施設サーベランス:病因と術式の検討2012

    • Author(s)
      鄭暁東, 布施昇男, 西田幸二, 井上幸次, 宮田和典, 木下茂, 天野史郎, 大橋裕一
    • Organizer
      角膜カンファランス2012
    • Place of Presentation
      東京
    • Year and Date
      20120223-25
  • [Presentation] マウス表皮細胞から形質転換した角膜上皮様細胞の免疫組織学的検討2012

    • Author(s)
      小林剛, 林康人, 渡部成美, 白石敦, 大橋裕一
    • Organizer
      角膜カンファランス2012
    • Place of Presentation
      東京
    • Year and Date
      20120223-25
  • [Presentation] 角膜上皮創傷治癒過程におけるepiregulinの役割2012

    • Author(s)
      張媛, 林康人, 小林剛, 吉岡龍治, 白石敦, 大橋裕一
    • Organizer
      角膜カンファランス2012
    • Place of Presentation
      東京
    • Year and Date
      20120223-25
  • [Presentation] プロスタグランジン系点眼液の培養眼表面細胞毒性の比較試験2012

    • Author(s)
      渡部成美, 小林剛, 白石敦, 大橋裕一
    • Organizer
      角膜カンファランス2012
    • Place of Presentation
      東京
    • Year and Date
      20120223-25
  • [Presentation] In vivo confocal microscopic evaluation of corneal subbasal nerves in patients with diabetic retinopathy2011

    • Author(s)
      Oka N, Shiraishi A, Joko T, Ohashi Y
    • Organizer
      European Society of Opthalmology
    • Place of Presentation
      Geneva, Switzerland
    • Year and Date
      20110604-07

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Published: 2015-07-16  

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