Exploring pathophysiology of sepsis based on the mechanism of granulopoiesis regulated by the C/EBPbeta transcription factor

2014 Fiscal Year Final Research Report

• Project/Area Number: 23592678
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: SHIME NOBUAKI 京都府立医科大学, 医学(系)研究科(研究院), 客員講師 (00260795)
• Co-Investigator(Kenkyū-buntansha): HIRAI Hideyo 京都大学, 医学研究科, 講師 (50315933)
• Research Collaborator: SATAKE Sakiko 京都大学
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 好中球 / 分化/増殖 / C/EBPβ / 造血幹細胞 / 敗血症 / カンジダ

Outline of Final Research Achievements

CCAAT/enhancer-binding protein β(C/EBPβ) could play crucial roles on cellular differentiation and functional maintenance in various types of somatic cells. Granulocyte production increases in response to increased neutrophil demand following infectious stress. In this study, we investigated the role of C/EBPβ at hematopoietic stem cell levels in stress-induced granulopoiesis. C/EBPβ was upregulated at the protein level in all the five distinct subpopulations of granulopoiesis in a mouse candidemia model. Using a C/EBPβ knockout mouse model, the role of C/EBPβ in cell cycle acceleration and proliferation of hematopoietic stem/progenitors was also confirmed. These data suggest that C/EBPβ is a significant controlling factor involved in the efficient amplification of early granulocyte precursors during emergency granulopoiesis.

Free Research Field

救急医学