A study of tissue trauma with hemorrhagic shock induced remote organ failure.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592686
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Nippon Medical School
• Principal Investigator: OHIZUMI Akira 日本医科大学, 医学部, 助教 (20277502)
• Co-Investigator(Kenkyū-buntansha): ARAI Masatoku 日本医科大学, 医学部, 助教 (60267127) ; MASUNO Tomohiko 日本医科大学, 医学部, 講師 (00318528)
• Project Period (FY): 2011 – 2013
• Keywords: 外傷 / 遠隔臓器障害

Research Abstract

The aim of our study was to investigate the mechanism of multiple organ failure after soft tissue trauma. First, we produced an animal model of tissue trauma, and verified the validity of the model for the study of trauma. Our produced trauma model was a non-analgesic murine tissue trauma model, which was constructed by the dorsal subcutaneous implantation of minced donor syngeneic tissue-bone matrix (TBX) precisely adjusted to body weight and studyed after 21 hrs. Next, we investigated the effect of two-hit phenomenon (tissue trauma/sepsis) on gastrointestinal motility using our produced mode. Syngeneic TBX model was a highly controllable and reliable survival model of tissue injury, and two hit synergy occurred as gastrointestinal motility was only severely altered in the combined tissue trauma/sepsis model.