Characterization of the outer membrane protein complex in the Por secretion pathway

2014 Fiscal Year Final Research Report

• Project/Area Number: 23592715
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: SAIKI Keitarou 日本歯科大学, 生命歯学部, 講師 (30297973)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 歯周病 / タンパク分泌

Outline of Final Research Achievements

Porphyromonas gingivalis, the etiologic agent of chronic periodontitis, secretes C-terminal domain (CTD) proteins, which are involved in virulence and proliferation of this bacterium, via the Por secretion pathway (PorSP). PorSP is composed of steps such as passage through the outer membrane via the CTD-dependent Por secretion system (PorSS), cleavage of their CTDs by the CTD signal peptidase PG0026, glycolipid-conjugation, and anchorage to the outer cell membrane and/or the extracellular vesicles. PG0026 is one of the CTD proteins and secreted via PorSS, but undergoes no successive modifications. In this study, I found that PG0026 anchors to the cell surface by interacting with outer membrane protein PG27 that is involved in the glycolipid modification of CTD proteins, and functions in processing of other CTD proteins such as gingipains. This study also demonstrates the complex formation of the outer membrane proteins in PorSP.

Free Research Field

生化学