Differentiation of iPS cells into salivary glands

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592718
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Showa University
• Principal Investigator: MISHIMA KENJI 昭和大学, 歯学部, 教授 (50275343)
• Co-Investigator(Kenkyū-buntansha): SAITO Ichiro 鶴見大学, 歯学部, 教授 (60147634) ; INOUE Hiroko 日本薬科大学, 薬学部, 准教授 (50367306) ; RYO Koufuchi 鶴見大学, 歯学部, 講師 (10298268)
• Project Period (FY): 2011 – 2013
• Keywords: 唾液分泌障害 / 再生医療 / iPS細胞

Research Abstract

Salivary gland hypofunction causes not only various oral diseases but also a decrease in quality of life by systemic diseases, such as aspiration pneumonia. However, to date, there is no efficient method to treat the patients with severe symptoms. The purpose of this study is to apply a regenerative medicine using induced pluripotent (iPS) cells for these patients. We established stromal cells from salivary glands of mice at embryonic day 11.5 and 13.5, respectively. iPS cells were differentiated into oral epithelium using LCA (large-cell aggregate)-SFEBq method. These iPS cells were cultured on the established feeder cells and specific gene expressions for salivary glands were detected by RT-PCR. Consequently, gene expressions of Aqp5, M3AchR, and beta2AdR, which are highly expressed in salivary glands, were increased in these iPS cells. Therefore, these results suggested that the present method could be applied for the differentiation of iPS cells into salivary gland cells.

Research Products

• [Journal Article] Identification of gene expression profile of neural crest-derived cells isolated from submandibular glands of adult mic (2014)
  • Author(s): Takahashi M, Suzawa T, Yamada A, Yamaguchi T, Mishima K, Osumi N, Maki K, Kamijo R
  • Journal Title: BBRC Volume: 446 Pages: 481-6
• [Journal Article] Downregulation of carbonic anhydrase IX promotes Col10a1 expression in chondrocytes (2013)
  • Author(s): Maruyama T, Miyamoto Y, Yamamoto G, Yamada A, Yoshimura K, Suzawa T, Takami M, Akiyama T, Hoshino M, Iwasa F, Ikumi N, Tachikawa T, Mishima K, Baba K, Kamijo R
  • Journal Title: PlosOne Volume: 8 Pages: e56984
• [Journal Article] Treatment of salivary gland hypofunction by transplantation with dental pulp cells (2013)
  • Author(s): Yamamura Y, Yamada H, Sakurai T, Ide F, Inoue H, Muramatsu T, Mishima K, Hamada Y, Saito I
  • Journal Title: Arch Oral Biol Volume: 58
• [Journal Article] Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma (2013)
  • Author(s): Hayashi S, Tanaka J, Okada S, Isobe T, Yamamoto G, Yasuhara R, Irie T, Akiyama C, Kohno Y, Tachikawa T, Mishima K
  • Journal Title: Exp Cell Res Volume: 319 Pages: 1220-8
• [Presentation] マウス唾液腺細胞におけるCD133陽性細胞の機能解 (2014)
  • Author(s): 田中準一、安原理佳、入江太朗、秋山知恵、河野葉子、美島健二
  • Organizer: 第13回日本再生医療学会総会
  • Place of Presentation: 京都
  • Year and Date: 2014-03-04