2013 Fiscal Year Final Research Report
Post-translational modifications of proteins from periodontpathic bacteria: role of glycosylation and phosphorylation
| Project/Area Number |
23592720
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Asahi University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIMURA Fuminobu 愛知学院大学, 歯学部, 教授 (50001962)
HASEGAWA Yoshiaki 朝日大学, 歯学部, 講師 (70460524)
INTO Takeshi 朝日大学, 歯学部, 講師 (10360918)
INOMATA Megumi 朝日大学, 歯学部, 助教 (40553798)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 歯周病関連細菌 / 蛋白質 / 翻訳後修飾 / 糖鎖修飾 / リン酸化修飾 / OmpA |
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| Research Abstract |
Post-translationally modified proteins from periodontopathic Porphyromonas gingivalis were analyzed. Besides OmpA-like proteins, three novel glycoproteins were found. These glycoproteins were involved in bacterial growth and biofilm formation. OmpA-like proteins were effectively isolated using a lectin column. In addition, at least six novel phosphorylated proteins were identified after separation using an affinity chromatography column.
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