2013 Fiscal Year Final Research Report
Rotavirus infection to oral epithelial cells
| Project/Area Number |
23592778
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | ロタウイルス / 上皮細胞 |
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| Research Abstract |
Rotavirus is a leading cause of the infant virus-related diarreha. The main target of rotavirus infection is intestinal epithelial cells. The purpose of this study is to elucidate whether rotavirus can infect to oral epithelial cells. For this purpose, oral squamous cell carcinoma-derived cell lines were selected. The infectivity of rotavirus was examined by both real-time PCR and immunofluoresence cell staining. The results clearly indicated that rotavirus can infect oral epithelial cells. Using intestinal adenocarcinoma cell line, HT-29, the production of type III interferon was also examined. The results indicated that rotavirus infection leads to type III interferon production mainly through NF-kB pathway. Rotavirus infection also leads to the production of type I interferon. The comparison between type I and type III interferons revealed that type III interferon was produced much higher amount but the anti-viral effect was much profound in type I interferon.
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