Establishment of in situ bone regenerative technology to induce mature cortical bone formation containing osteons

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592913
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: The University of Tokyo
• Principal Investigator: KANNNO Yuki 東京大学, 医学部附属病院, 助教 (80451813)
• Co-Investigator(Kenkyū-buntansha): SAIJO Hideto 東京大学, 医学部附属病院, 講師 (80372390) ; HOSHI Kazuto 東京大学, 医学部附属病院, 准教授 (30344451) ; FUJIHARA Yuko 東京大学, 医学(系)研究科(研究院), 特任助教 (50466744) ; KIKUCHI Masanori 独立行政法人, 物質材料機構, リーダー (00354267)
• Project Period (FY): 2011 – 2013
• Keywords: オステオン / 皮質骨誘導型人工骨 / 成長因子 / サイトカイン / in situ骨再生技術 / 吸収性メッシュトレー

Research Abstract

The purpose of this study is to clarify the mechanism of osteons formation and regeneration that has never been studied and in vivo osteons formation and regeneration mechanism using novel biomaterials technologies. Therefore, we establish the in situ bone regeneration techniques to induce maturation bone matrix formation that was difficult until now. Thus, we examined the utility of cortical bone induced artificial bone with the addition of growth factors or cytokines by using the experimental dog (Beagle) model which found osteons structure in the same as human.
Furthermore, we prepared in situ regenerative bone tissue, which placed in a new absorbable mesh tray cortical bone induced artificial bone, and implanted in beagle bone defect model to verify their usefulness.

Research Products

• [Journal Article] Evaluation of in vivo migration of chondrocytes from tissue-engineered cartilage that was subcutaneously transplanted in mouse model (2013)
  • Author(s): Matsuyama M, Fujihara Y, Inaki R, Nishizawa S, Nagata S, Takato T, Hoshi K.
  • Journal Title: OJRM Volume: 2(4) Pages: 93-98
• [Journal Article] Bone and cartilage repair by transplantation of induced pluripotent stem cells in murine joint defect model (2013)
  • Author(s): Uto S, Nishizawa S, Takasawa Y, Asawa Y, Fujihara Y, Takato T, Hoshi K.
  • Journal Title: Biomed Res Volume: 34(6) Pages: 281-288
• [Journal Article] Usefulness of Agarose Mold as a Storage Container for Three-Dimensional Tissue-Engineered Cartilage (2013)
  • Author(s): Mori Y, Kanazawa S, Watanabe M, Suenaga H, Okubo K, Nagata S, Fujihara Y, Takato T, Hoshi K.
  • Journal Title: Materials and Sci Applications Volume: 4 Pages: 72-78
• [Journal Article] Hollow Fiber Module Applied for Effective Proliferation and Harvest of Cultured Chondrocytes (2013)
  • Author(s): Mori Y, Watanabe M, Nakagawa S, Asawa Y, Nishizawa S, Okubo K, Saijo H, Nagata S, Fujihara Y, Takato T, Hoshi K.
  • Journal Title: Materials Sci and Applications Volume: 4 Pages: 62-67
• [Journal Article] Clinicalresentation of epignathus teratoma with cleft palate; and duplication of cranial base, tongue, mandible, and pituitary gland (2013)
  • Author(s): Maeda Y, Suenaga H, Sugiyama M, Saijo H, Hoshi K, Mori Y, Takato T.
  • Journal Title: J Craniofac Surg Volume: 24(4) Pages: 1486-1491
• [Journal Article] The junction between hyaline cartilage and engineered cartilage in rabbits (2013)
  • Author(s): Komura M, Komura H, Otani Y, Kanamori Y, Iwanaka T, Hoshi K, Tsuyoshi T, Tabata Y.
  • Journal Title: Laryngoscope Volume: 123(6) Pages: 1547-1551
• [Journal Article] Real-time in situ three-dimensional integral videography and surgical navigation using augmented reality : a pilot study (2013)
  • Author(s): Suenaga H, Hoang Tran H, Liao H, Masamune K, Dohi T, Hoshi K, Mori Y, Takato T.
  • Journal Title: Int J Oral Sci Volume: 5(2) Pages: 98-102
• [Journal Article] Application of floating cells for improved harvest in human chondrocyte culture (2012)
  • Author(s): Yonenaga K, Nishizawa S, Fujihara Y, Asawa Y, Kanazawa S, Nagata S, Takato T, Hoshi K.
  • Journal Title: Biomed Res Volume: 33(5) Pages: 281-9
• [Journal Article] Evaluation of the implant type tissue-engineered cartilage by scanning acoustic microscopy (2012)
  • Author(s): Tanaka Y, Saijo Y, Fujihara Y, Yamaoka H, Nishizawa S, Nagata S, Ogasawara T, Asawa Y, Takato T, Hoshi K.
  • Journal Title: J Biosci Bioeng Volume: 113(2) Pages: 252-7
• [Journal Article] BMP-2 embedded atelocollagen scaffold for tissue-engineered cartilage cultured in the medium containing insulin and triiodothyronine--a new protocol for three-dimensional in vitro culture of human chondrocytes (2012)
  • Author(s): Ko EC, Fujihara Y, Ogasawara T, Asawa Y, Nishizawa S, Nagata S, Takato T, Hoshi K.
  • Journal Title: Tissue Eng Part C Methods Volume: 18(5) Pages: 374-86
• [Journal Article] A novel method for designing and fabricating custom-made artificial bones (2011)
  • Author(s): Saijo H, Kanno Y, Mori Y, Suzuki S, Ohkubo K, Chikazu D, Yonehara Y, Chung UI, Takato T.
  • Journal Title: Int J Oral Maxillofac Surg Volume: 40(9) Pages: 955-60
• [Journal Article] Extraction of 6 molars under general anesthesia in patient with fibrodysplasia ossificans progressiva (2011)
  • Author(s): Mori Y, Susami T, Haga N, Tamura K, Kanno Y, Saijo H, Takato T.
  • Journal Title: J Oral Maxillofac Surg Volume: 69(7) Pages: 1905-10
• [Journal Article] Macrophage-inducing fasl on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration
  • Author(s): Fujihara Y, Takato T, Hoshi K.
  • Journal Title: Stem Cells Volume: (in press)
• [Journal Article] Recent trends of cartilage regenerative medicine and its application to the oral and maxillofacial surgery
  • Author(s): Hoshi K, Fujihara Y, Asawa Y, Nishizawa S, Kanazawa S, Sakamoto T, Watanabe M, Ogasawara T, H S, Mori Y, Takato T.
  • Journal Title: Oral Sci Int Volume: (in press)
• [Journal Article] Fabrication of Stereotyped Beta-Tricalcium-Phosphate Blocks into a Conjugated Structure using Mesenchymal Stem Cell Sheets Prepared in Temperature-Responsive Culture Dishes
  • Author(s): Mori Y, Fujihara Y, Misawa M, Inoue H, Inaki R, Suenaga H, Okubo K, Saijo H, Takato T, Hoshi K.
  • Journal Title: J of Hard Tissue Biology Volume: (in press)
• [Journal Article] Submucous cleft palate : variations in bony defects of the hard palate
  • Author(s): Mori Y, Hoshi K, Takato T, Takahashi M, Hirano Y, Kanno Y, Ohkubo K, Saijo H.
  • Journal Title: Br J Oral Maxillofac Surg Volume: 51(8) Pages: e220-e223
• [Presentation] 自己組織化ペプチドを活用した次世代再生軟骨組織の研究開発 (2014)
  • Author(s): 星和人
  • Organizer: 第13回日本再生医療学会総会
  • Place of Presentation: 国立京都国際会館, 京都
  • Year and Date: 2014-03-04
• [Presentation] 軟骨の再生・再建-軟骨の生態学的研究- (2014)
  • Author(s): 星和人
  • Organizer: 第27回日本軟骨代謝学会
  • Place of Presentation: 京都府医師会館, 京都
  • Year and Date: 2014-03-01
• [Presentation] 足場素材を導入した3次元再生軟骨の研究開発 (2013)
  • Author(s): 星和人
  • Organizer: 第16回ヒューマンサイエンス総合研究ワークショップ
  • Place of Presentation: 東京
  • Year and Date: 2013-12-26
• [Presentation] 生分解性ポリマーを用いた軟骨再生医療の研究開発 (2013)
  • Author(s): 星和人
  • Organizer: 第30回高分子研究会講座
  • Place of Presentation: 東京大学生産技術研究所, 東京
  • Year and Date: 2013-11-27
• [Presentation] Clinical Application of Tissue-engineered Cartilage in Craniofacial Areas (2013)
  • Author(s): Kazuto Hoshi
  • Organizer: 21st International Conference on Oral and Maxillofacial Surgery
  • Place of Presentation: Palau de Congressos de Cataluña Barcelona, Spain
  • Year and Date: 2013-10-13
• [Presentation] Cartilage Regeneration and Usefulness of Medication (2013)
  • Author(s): Kazuto Hoshi
  • Organizer: International Cartilage Repair Society 2013
  • Place of Presentation: Swissotel Gran Efes, Izmir, Turkey
  • Year and Date: 2013-09-17
• [Presentation] Optimal Combinations of Scaffolds and Growth Factors for Tissue Engineering of Cartilage (2013)
  • Author(s): Kazuto Hoshi
  • Organizer: Osteoarthritis Research Society International World Congress
  • Place of Presentation: Marriott Philadelphia Downtown, Philadelphia, USA
  • Year and Date: 2013-04-20
• [Presentation] Chondrocytes suppress tissue reactions in tissue-engineered cartilage through induction of immune privilege (2012)
  • Author(s): Fujihara Y, Takato T, Hoshi K.
  • Organizer: 3rd TERMIS World Congress 2012
  • Place of Presentation: Vienna, Austria, Hofburg Congress Center
  • Year and Date: 20120905-08
• [Presentation] 片側性唇顎口蓋裂患者に対し, チタンメッシュを併用し, インプラント治療を行った1例 (2011)
  • Author(s): 菅野勇樹, 西條英人, 斎藤健太郎, 古賀陽子, 杉山円, 安部貴大, 瀬戸一郎, 森良之, 高戸毅
  • Organizer: 第15回日本顎顔面インプラント学会総会・学術大会
  • Year and Date: 20111203-04