2013 Fiscal Year Final Research Report

A base maintenance study to perform security and reliable cell treatment that used a human marrow origin mesenchymal cell.

Research Project

Project/Area Number	23592923
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Surgical dentistry
Research Institution	Hiroshima University
Principal Investigator	OKAMOTO KOSEI 広島大学, 病院(歯), 病院助教 (40423371)
Co-Investigator(Kenkyū-buntansha)	KURIHARA Hidemi 広島大学, 医歯薬保健学研究院(歯), 教授 (40161765) OKAMOTO Tetsuji 広島大学, 医歯薬保健学研究院(歯), 教授 (00169153)
Project Period (FY)	2011 – 2013
Keywords	歯学 / 再生医学
Research Abstract	With a mesenchyma system stem cell (MSC) derived from establishment marrow, I examined it in a serum-free medium for embryonic stem cells. In CD90 (Thy-1), CD105 (Endogrlin) which was a marker gene of the MSC, and gene Oct3/4, Nanog where expression was accepted with an undifferentiated human embryonic stem cell, expression was recognized in the MSC of which condition that added FGF-1, FGF-2, LIF, activin A and heparin in POWEREDBY10 or hESF7 nutrient medium. In addition, expression was accepted in the MSC which I cultured in hESF9 and hESF10 nutrient medium equally. Possibility to become the index of the undifferentiation voltinism of the MSC was suggested.