Immunohistochemical study of the glial function on the plastisity of medullary notiception

2013 Fiscal Year Final Research Report

• Project/Area Number: 23592978
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hokkaido University
• Principal Investigator: TAKUMA Shigeru 北海道大学, 歯学研究科(研究院), 助教 (60360921)
• Project Period (FY): 2011 – 2013
• Keywords: グリア細胞 / ミクログリア / アストロサイト / 延髄 / 浮遊切片 / 蛍光抗体法

Research Abstract

To investigate the role of the glial function on the plastisity of the spinal trigeminal nucleus, immunohistochemical staining were performed on the horizontal slices of the rat medulla oblongata. At first, immunoenzyme technique were performed on the paraffin sections. Thereafter, immunofluorescence on the free-float sections were performed to obtain more efficient and valued results. After perfusion fixation with paraformaldehyde under deep anesthesia, the medulla oblongata were extirpated from neonatal rats. Thereafter, 50-100 micron-thick unflozen holizontal slices of the medulla oblongata were made, then stained with fluorescent antibody method. As a result, high intensity fluorescence signals of microglia(IbaI) or astrosyte(GFAP) were observed in the spinal trigeminal nucleus. Thus, the usefulness of this method for the investigation of the glial functions in the spinal trigeminal nucleus was suggested.