Enrichment of the tooth constitution cell derived from human iPS cells using an original technique

2013 Fiscal Year Final Research Report

• Project/Area Number: 23593038
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Kagoshima University
• Principal Investigator: HASEGAWA Hiroko 鹿児島大学, 医歯(薬)学総合研究科, 客員研究員 (00295271)
• Co-Investigator(Kenkyū-buntansha): SATO Masahiro 鹿児島大学, 医用ミニブタ先端開発研究センター, 教授 (30287099) ; SAITOH Issei 新潟大学, 医歯学総合研究科, 准教授 (90404540) ; YAMASAKI Youichi 鹿児島大学, 医歯学総合研究科, 教授 (30200645) ; HAYASAKI Haruaki 新潟大学, 医歯学総合研究科, 教授 (60238095) ; NOGUCHI Hirofumi 独立行政法人国立病院機構, 千葉東病院研究センター, 研究員 (50378733)
• Project Period (FY): 2011 – 2013
• Keywords: iPS細胞 / ameloblast / 遺伝子工学

Research Abstract

We obtained a primary culture of teratoma cells from a nude mouse that was transplanted with a pT-ARIP introduced in an iPS cell. We attempted to detect cells responsible for tooth development based on the expression of the fluorescent gene; however, the cells were not detected.. In the process in which a teratoma was formed, repression of gene expression likes gene silencing may have started the introduced gene. Hence, RT-PCR analysis of the primary teratoma cell culture obtained from iPS cells before transgenesis was conducted. The expression of OCT3/4, NANOG, NESTIN, OC (osteocalcin), DMP, DSPP, and BGP was observed. Moreover, the existence of AMELX was confirmed by immunohistological analysis.

Research Products

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• [Journal Article] Expression analysis of aα-1, 3-galactosyltransferase, an enzyme that creates xenotransplantation-relatedα-Gal epitope, in pig preimplantation embryos (2012)
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• [Journal Article] Successful suppression of endogenousα-1,3-galactosyltransferase expression by RNA interference in pig embryos generated in vitro (2012)
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• [Journal Article] In vivo gene transfer in mouse preimplantation embryos after intraoviductal injection of plasmid DNA and. Systems Biology in Reproductive Medicinesubsequent in vivo electroporation (2012)
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• [Journal Article] Determination of the optimal concentration of several selective drugs useful for generating multi-transgenic porcine embryonic fibroblasts (2012)
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• [Presentation] トランスポゾンPiggyBacシステムによる複数遺伝子のブタ細胞への同時導入 (2013)
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