2013 Fiscal Year Final Research Report
Molecular mechanisms of BSP gene transcription on cementblast
| Project/Area Number |
23593050
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
YAMAUCHI Masato 神奈川歯科大学, 歯学研究科(研究院), 助教 (30230311)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Masahiro 東北大学, 歯学研究科(研究院), 教授 (40215562)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 骨シアロ蛋白質 / Ｒｕｎｘ２ / ＢＭＰ－２ |
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| Research Abstract |
Bone Sialoprotein(BSP) is a tissue-specific marker of cementblast cells which has shown to be transformed from Hertwig's epithlial cells.To elucidate the mechanisms of gene transcription,3 putative osteoblast specific element 2 consensus motif(OSE2-1~OSE2-3) were investigated the ability of Runx2 to interact with cognate cis-acting elements. Forced expression of Runx2 induced 14 fold enhancement of transcription activity observed with the 6 x tandem repeats of OSE2-2 and 3 fold enhancement with that of OSE2-3, whereas 60% significant reduction was observed with that of OSE2-1. Chromatin immunorecipitation analyses revealed Runx2 were significantly higher recruited to OSE2-2 than to other OSE2s in vivo. BMP-2 increased Runx2 binding to these OSE2s and its transcription, whereas obvious reduction of OSE2-2 transcription induced by TGF-beta 1. These data suggested that the coordinated regulation of chromatin on respective OSE2s with Runx2 were modulated by TGF-beta/BMP signals.
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[Journal Article] ADAMT SL6beta protein resucues fibrillin-1 microfibril disorder in a Marfan syndrome mouse model through the promotion of fibrillin-1 assembly2011
Author(s)
Saito M, Kurokawa M, Oda M, Oshima M, Tsutsui K, Nakao K, Ogawa M, Manabe R, Suda N, Ganjargal G, Hada Y, Noguchi T, Teranaka T, Sekiguchi K, Yoneda T, Tsuji T
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Journal Title
J Biol Chem
Volume: 286(4)
Pages: 38602-38613
DOI
Peer Reviewed
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