2013 Fiscal Year Final Research Report
Mechanisms of spindle elongation controlling the chromosome segregation during anaphase: quantitative measurement of forces generated by the mitotic spindle
| Project/Area Number |
23612006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Mechanobiology
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 細胞骨格 / 紡錘体 / 染色体分配 |
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| Research Abstract |
The process of partitioning chromosomes into daughter cells during cell division is mediated by a bipolar spindle of proper size and shape. We planned to investigate the underlying mechanisms of spindle elongation machinery by quantitative force measurements. The main results of this project are summarized as follows: (1) We developed the technique for real-time observation to analyze dynamics of chromokinesin within meiotic spindle. (2) Using micromanipulation technique, we found the parameters controlling the size of vertebrate meiotic spindle. (3) We revealed the micromechanics of meiotic spindle along the pole-to-pole axis. (4) We succeeded in quantitatively measuring forces generated by anaphase cells.
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