Genome wide screening of genomic DNA demethylation factors by genetic method.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23616002
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: epigenetics
• Research Institution: Hiroshima University
• Principal Investigator: KIKUCHI Yutaka 広島大学, 理学(系)研究科(研究院), 教授 (20286438)
• Co-Investigator(Kenkyū-buntansha): HOZUMI Shunya 広島大学, 大学院理学研究科, 助教 (10597222)
• Project Period (FY): 2011 – 2013
• Keywords: ゼブラフィッシュ / 変異体 / スクリーニング / 脱メチル化 / 発生 / 再生

Outline of Final Research Achievements

Epigenetics is a regulatory system for gene expression independent of genomic DNA sequence. Genomic DNA methylation is one of epigenetics system, but the mechanisms of DNA demethylation remain to be elucidated in animal cells. To uncover the DNA demethylation systems in animal cells, I tried to screen new zebrafish mutants that have defects in DNA demethylation, but my research goal was not achieved. In addition to mutant screening, I found that both activation-induced cytidine deaminase and methyl CpG-binding domain protein 4 do not function in DNA demethylation, and the level of DNA methylation is dynamically changed during development and caudal fin regeneration in zebrafish.

Free Research Field

分子生物学