2014 Fiscal Year Final Research Report
Establishment of hematopoietic differentiation system of human iPS cells in microenvironment of ovine fetuses
| Project/Area Number |
23618005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Regenerative medicine
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| Research Institution | Utsunomiya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUKUMORI Rika 宇都宮大学, 農学部, 助教 (60721694)
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| Co-Investigator(Renkei-kenkyūsha) |
HANAZONO Yutaka 自治医科大学, 医学部, 教授 (70251264)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Keywords | iPS細胞 / 分化誘導 / 造血幹細胞 / 大動物 / 胎子 |
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| Outline of Final Research Achievements |
We found that hematopoietic engraftment was only observed when ES cell-derivatives at the early differentiation stage were transplanted into fetal livers at 51-67 days of gestation. However, teratoma formation with mature monkey tissue structures was observed following transplantation of undifferentiated ES cells into the fetal subcutaneous tissue, but not into the liver, and at 43-50 days of gestation, but not at 51-67 days. Stem cell factor and other many factors man be concerned in the hematopoietic differentiation. Pre-conditioning by maternal administration of busulfan before in utero transplantation of monkey ES cells enhanced hematopoietic engraftments in sheep. And then, human iPS cells were transplanted into ovine fatal liver to deifferentiate into hematopoietic tissue.
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| Free Research Field |
幹細胞学
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