Elucidation of neuronal basis of anxiety behavior

2012 Fiscal Year Final Research Report

• Project/Area Number: 23650193
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Kitasato University
• Principal Investigator: TAKAHASHI Masami 北里大学, 医学部, 教授 (10318826)
• Co-Investigator(Renkei-kenkyūsha): MIYAOKA Hitoshi 北里大学, 医学部, 教授 (40209862) ; ITAKURA Makoto 北里大学, 医学部, 講師 (30398581) ; AZUMA Sadahiro 北里大学, 医学部, 助教 (80348507) ; YAMAMORI Saori 北里大学, 医学部, 助教 (30464803)
• Project Period (FY): 2011 – 2012
• Keywords: SNAP-25 / ピロカルピン / てんかん / 不安様行動 / モノアミン / BDNF

Research Abstract

Neuronal mechanism of anxiety behavior observed after having repeated epileptic seizures are investigated using two mouse models. Catecholamine was not involved, but some changes in brain structure that appear within three days after repeated epileptic seizures are responsible for the development of anxiety-related behavior. From immunoblot analysis of brain homogenate, expression of BDNF was paralleled with the expression of anxiety behavior.

Research Products

• [Journal Article] Protein phosphatase 2A dephosphorylates SNAP-25 through two distinct mechanisms in mouse brain synaptosomes (2013)
  • Author(s): Iida Y, Yamamori S, Itakura M, Miyaoka H, Takahashi M
  • Journal Title: Neurosci Res Volume: 75 Pages: 184-189
  • Peer Reviewed
• [Journal Article] Synaptosomal-associated protein 25 mutation induces immaturity of the dentate granule cells of adult mice (2013)
  • Author(s): Ohira K, Kobayashi K, Toyama K, Nakamura HK, Shoji H, Takao K, Takeuchi R, Yamaguchi S, Kataoka M, Otsuka S, Takahashi M, Miyakawa T
  • Journal Title: Mol Brain Volume: 6 Pages: 12
  • Peer Reviewed
• [Journal Article] Accumulation of-synuclein triggered by presynaptic dysfunction (2012)
  • Author(s): Nakata Y, Yasuda T, Fukaya M, Yamamori S, Itakura M, Nihira T, Hayakawa H, Kawanami A, Kataoka M, Nagai M, Sakagami H, Takahashi M, Mizuno Y, and Mochizuki H
  • Journal Title: J Neurosci Volume: 32 Pages: 17186-17196
  • Peer Reviewed
• [Journal Article] A Single Amino Acid Mutation in SNAP-25 Induces Anxiety-Related Behavior in Mouse (2011)
  • Author(s): Kataoka M, Yamamori S, Suzuki E, Watanabe S, Sato T, Miyaoka H, Azuma S, Ikegami S, Kuwahara R, Suzuki-Migishima R, Nakahara Y, Nihonmatsu I, Inokuchi K, Katoh-Fukui Y, Yokoyama M, Takahashi M
  • Journal Title: PLoS ONE Volume: 6(9) Pages: e25158
  • Peer Reviewed
• [Presentation] Stress induced phosphorylation of SNAP-25 (2012)
  • Author(s): Yamamori S, Sugaya D, Iida Y, Kokubo H, Itakura M, Suzuki E, Kataoka M, Miyaoka H, Takahashi M
  • Organizer: 北米神経科学会大会
  • Place of Presentation: ニューオリンズ(米国)
  • Year and Date: 2012-10-17
• [Presentation] Calcium-binding protein calretinin is highly expressed in CA3 neurons of epileptized brain in pilocarpine model (2012)
  • Author(s): Ohkido T, Itakura M, Yamamori S, Watanabe S, Miyaoka H, Takahashi M
  • Organizer: Calcium-binding protein calretinin is highly expressed in CA3 neurons of epileptized brain in pilocarpine model
  • Place of Presentation: Kobe
  • Year and Date: 2012-09-30
• [Presentation] SNAP-25リン酸化部位変異マウスが示す異常行動の一部には、カテコールアミンが関与する (2012)
  • Author(s): 飯田諭宜、小久保宏俊、山森早織、板倉誠、渡辺滋、宮岡等、高橋正身
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-19
• [Presentation] ピロカルピンによって誘発されたてんかん重積後に、NMDA 型受容体はサブユニット特異的に減少する (2012)
  • Author(s): 板倉誠、大城戸太朗、菅谷津貴子、山森早織、深谷昌弘、阪上洋行、高橋正身
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-18
• [Presentation] 小島正己げっ歯類 Bdnf アンチセンス RNA の同定と機能 (2012)
  • Author(s): 熊ノ郷晴子、永井利幸、清末和之、高橋正身
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-18
• [Presentation] 神経栄養因子プロセシング障害マウスの解析 (2012)
  • Author(s): 小島正己、熊ノ郷晴子、永井利幸、高橋正身、清末和之
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-09-18
• [Presentation] SNARE タンパク質SNAP-25 の脱リン酸化の機構 (2011)
  • Author(s): 飯田諭宜、山森早織、中屋千恵美、板倉誠、宮岡等、高橋正身
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 山代温泉
  • Year and Date: 2011-09-27
• [Presentation] 不安様行動発現における、BDNF 関与の可能性 (2011)
  • Author(s): 大城戸太朗、飯塚健、板倉誠、大塚新太朗、渡邉崇嗣、飯田諭宜、高橋正身
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 山代温泉
  • Year and Date: 2011-09-27
• [Presentation] SNAP-25のリン酸化は生後発達期のてんかんと不安様行動の発症の抑制に関与する (2011)
  • Author(s): 大塚新太朗、山森早織、渡辺滋、鈴木映二、斉藤正範、宮岡等、高橋正身
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-16
• [Remarks]
  • URL: http://web.med.kitasato-u.ac.jp/edures/biochem/