2012 Fiscal Year Final Research Report
Screening of voltage-sensitive proteins using Voltage-Sensing Phosphatase, VSP
| Project/Area Number |
23650210
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKOCHI Yoshifumi 大阪大学, 医学系研究科, 助教 (90435818)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 生理学 / 生体分子 / 蛋白質 / 電位センサー / イオンチャネル |
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| Research Abstract |
We aimed to develop a functional screening system for novel voltage-sensitive membrane protein modules through making a chimeric protein with voltage-sensing phosphatase. First, we identified Ci-VSP as the most suitable among VSP orthologsfor developing the system, since readout of PI(4,5)P2 phosphatase activity was most remarkable. Ci-VSPwas remodeled into a form in which enzyme activity was more remarkablyenhanced by membrane voltage. Other voltage sensors were fused to the cytoplasmic region of Ci-VSP to acquire chimeric voltage sensing phosphatases.
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