Comprehensive analysis of in vivo substrates of protein kinase C

2012 Fiscal Year Final Research Report

• Project/Area Number: 23650214
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Fusional basic brain science
• Research Institution: The University of Tokyo
• Principal Investigator: AIBA Atsu 東京大学, 大学院・医学系研究科, 教授 (20271116)
• Project Period (FY): 2011 – 2012
• Keywords: 脳分子プロファイリング

Research Abstract

Protein kinase C (PKC) γ is involved in cerebellar functions such as motor coordination and elimination of climbing fiber-Purkinje cell synapses. Molecular mechanisms of PKCγ-regulating cerebellar processes are elusive, due to the heretofore undescribed in vivo substrates of PKCγ. In this study, a series of candidate molecules for PKCγ substrates in mouse cerebellum was identifiedby quantitative phospho-proteomic analysis.

Research Products

• [Journal Article] Ras-related C3 botulinum toxin substrate 1 (RAC1) regulates glucose-stimulated insulin secretion via modulation of F-actin (2013)
  • Author(s): Asahara S, Shibutani Y, Teruyama K, InoueHY, Kawada Y, Etoh H, Matsuda T,Kimura-Koyanagi M, Hashimoto N, Sakahara M, Fujimoto W, Takahashi H, Ueda S, Hosooka T, Satoh T, Inoue H, Matsumoto M, Aiba A, Kasuga M, Kido Y
  • Journal Title: Diabetologia
  • DOI: DOI:10.1007/s00125-013-2849-5
  • Peer Reviewed
• [Journal Article] Guidepost neurons for the lateral olfactory tract: Expression of metabotropic glutamate receptor 1 and innervation by glutamatergic olfactory bulb axons (2012)
  • Author(s): Hirata T, Kumada T, Kawasaki T, Furukawa T, Aiba A, Conquet F, Saga Y, Fukuda A
  • Journal Title: Developmental Neurobiology Volume: 72 Pages: 1559-1576
  • DOI: DOI:10.1002/dneu.22030
  • Peer Reviewed
• [Journal Article] Functional coupling of the metabotropic glutamate receptor, InsP_3 receptor and L-type Ca^2+ channel in mouse CA1 pyramidal cells (2012)
  • Author(s): Kato HK, Kassai H, Watabe AM, Aiba A, Manabe T
  • Journal Title: Journal of Physiology Volume: 590 Pages: 3019-3034
  • DOI: DOI:10.1113/jphysiol.2012.232942
  • Peer Reviewed
• [Journal Article] Cdc42 is required for chondrogenesis and interdigital programmed cell death during limb development (2012)
  • Author(s): Aizawa R, Yamada A, Suzuki D, Iimura T, Kassai H, Harada T, Tsukasaki M, Yamamoto G, Tachikawa T, Nakao K, Yamamoto M, Yamaguchi A, Aiba A, Kamijo R
  • Journal Title: Mechanisms of Development Volume: 129 Pages: 38-50
  • DOI: DOI:10.1016/j.mod.2012.02.002
  • Peer Reviewed
• [Journal Article] In vivo imaging visualizes discoid platelet aggregations without endothelium disruption and implicates contribution of inflammatory cytokine and integrin signaling (2012)
  • Author(s): Nishimura S, Manabe I, Nagasaki M, Kakuta S, Iwakura Y, Takayama N, Ooehara J, Otsu M, Kamiya A, Petrich BG, Urano T, Kadono T,Sato S, Aiba A, Yamashita H, Sugiura S, Kadowaki T, Nakauchi H, Eto K, Nagai R
  • Journal Title: Blood Volume: 119 Pages: e45-56
  • DOI: DOI:10. 1182/blood-2011-09-381400
  • Peer Reviewed
• [Journal Article] Suppression of a neocortical potassium channel activity by intracellular amyloid-ss and its rescue with Homer1a (2011)
  • Author(s): Yamamoto K, Ueta Y, Wang L, Yamamoto R, Inoue N, Inokuchi K, Aiba A, Yonekura H, Kato N
  • Journal Title: Journal of Neuroscience Volume: 31 Pages: 11100-11109
  • Peer Reviewed
• [Presentation] 中枢神経系において恒常的活性化型mTORを発現するマウスの作製と解析 (2012)
  • Author(s): 饗場篤
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-13
• [Presentation] Genetic dissection of mGluR1 function in cerebellar Purkinje cells (2011)
  • Author(s): Aiba A
  • Organizer: 7th International Meeting on Metabotropic Glutamate Receptors
  • Place of Presentation: Grande Albergo Capotaormina (Italy)
  • Year and Date: 2011-10-03
• [Book] 「mTORシグナルによる脳機能の調節と破綻」細胞工学 (2012)
  • Author(s): 葛西秀俊,饗場篤
  • Total Pages: 1355-1359
  • Publisher: 学研メディカル秀潤社
• [Remarks]
  • URL: http://lar.cdbim.m.u-tokyo.ac.jp/