Generation of a transgenic mouse which can be used for the in vivo detection of CREB phosphorylatio

2012 Fiscal Year Final Research Report

• Project/Area Number: 23650231
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: University of Toyama
• Principal Investigator: ISHIMOTO Tetsuya 富山大学, 大学院・医学薬学研究部(医学), 助教 (40397170)
• Project Period (FY): 2011 – 2012
• Keywords: トランスジェニックマウス / ルシフェラーゼ

Research Abstract

In 23rdfiscal year, we succeeded in developing the CREB phosphorylation detecting system using anesthesized mouse by adjasting the timing and amount of anesthesia. In this year, we succeeded in observing CREB phosphorylation in non-anesthesized mouse. We confirmed acute adminidtration of Fluoxetine up-regulated the light intensity from mouse brain which indicated the CREB phosphorylation. And we also confirmed the up-regulation of CREB phosphorylation after Fluoxetine treatment by westernblotting.

Research Products

• [Journal Article] Measuring CREB activation using bioluminescent probes that detect KID-KIX interaction in living cells (2012)
  • Author(s): Ishimoto, T., Mano, H., Ozawa, T., Mori, H.
  • Journal Title: Bioconjug. Chem Volume: 23 Pages: 923-932
  • Peer Reviewed
• [Journal Article] Regulation of gene expression in retrovirus vectors by X-ray and proton beam radiation with artificially constructed promoters. (2012)
  • Author(s): Ogawa, R., Morii, A., Watanabe, A., Cui, Z. G., Kagiya, G.,Fukuda, S., Kume, K., Hasegawa, T., Hatashita, M., Izumi, H., Ishimoto, T.,Feril, L. B.
  • Journal Title: J. Gen. Med. Volume: 14 Pages: 316-327
  • Peer Reviewed
• [Journal Article] Real-time monitoring of actin polymerization in living cells using split luciferase (2011)
  • Author(s): Ishimoto, T., Ozawa, T., Mori,H.
  • Journal Title: Bioconjug. Chem Volume: 22 Pages: 1136-1144
  • Peer Reviewed
• [Journal Article] Bioluminescence imaging of Arc expression enables detection of activity-dependent and plastic changes in the visual cortex of adult mice. (2011)
  • Author(s): Izumi,H., Ishimoto, T., Yamamoto, H., Nishijo,H., Mori, H.
  • Journal Title: Brain Struct. Funct Volume: 216 Pages: 91-104
  • Peer Reviewed
• [Journal Article] Purification of enzymatically inactive peptidylarginine deiminase type 6 from mouse ovary that reveals hexameric structure different from other dimeric isoforms. (2011)
  • Author(s): Taki, H., Gomi, T., Knuckley, B., Thompson, P. R., Vugrek, O., Hirata, K., Miyahara, T., Shinoda, K., Hounoki, H., Sugiyama, E., Usui, I., Urakaze, M., Tobe,K., Ishimoto, T., Inoue, R., Tanaka, A., Mano, H., Ogawa, H., Mori, H.
  • Journal Title: Adv. Biosci. Biotech Volume: 2 Pages: 304-310
  • Peer Reviewed
• [Presentation] Analysis of Newly Identified Paranodal Protein BCAS1 in the Schwann cell (2012)
  • Author(s): 石本哲也、井上 蘭、小池正人、内山安男、森 寿
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 20121200
• [Presentation] Detection of CREB activation in living cells using a bioluminescence-based method. (2011)
  • Author(s): Ishimoto T., Mano H., Ozawa T., and Mori H.
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20111213-17
• [Presentation] 発光蛋白質を用いたCREB活性化の計測 (2011)
  • Author(s): 石本哲也,眞野寛生,小澤岳昌,森 寿
  • Organizer: CRESTシンポジウム「新しい計測で生命に迫る 」
  • Place of Presentation: 東京
  • Year and Date: 20111212-13
• [Presentation] Monitoring of CREB phosphorylation in live cells using split luciferase technique (2011)
  • Author(s): Ishimoto T., Mano H., Ozawa T., and Mori H
  • Organizer: Society For Neuroscience's 41th annual meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20111112-16
• [Presentation] 生物発光を用いたCREBリン酸化検出 (2011)
  • Author(s): 石本哲也,眞野寛生,小澤岳昌,森 寿
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横 浜
  • Year and Date: 20110914-17
• [Presentation] Detection of CREB phosphorylation in live cells using a bioluminescence based method. Ishimoto T (2011)
  • Author(s): Mano H., Ozawa T., and Mori H.
  • Organizer: The 6th International conference of Neurons and Brain Diseases
  • Place of Presentation: Toyama.
  • Year and Date: 20110803-05