STUDY ON ROLE OF INTRACELLULAR FORCE TRANSMISSION VIA LINC COMPLEX IN CELL RESPONSES

2012 Fiscal Year Final Research Report

• Project/Area Number: 23650250
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kawasaki University of Medical Welfare (2012); Tohoku University (2011)
• Principal Investigator: SAKAMOTO Naoya 川崎医療福祉大学, 医療技術学部, 准教授 (20361115)
• Project Period (FY): 2011 – 2012
• Keywords: バイオメカニクス / 生物生体工学 / 細胞核 / 細胞骨格 / 架橋タンパク質 / 細胞 内力学伝達

Research Abstract

In this study, the author investigated the mechanical role of nesprin, a component of linker of nucleus and cytoskeleton (LINC) in force transmission from actin filaments to the nucleus. Results showed that force transmission between actin filaments and nuclei via nesprin is required for stretch-induced morphological response of endothelial cells, suggesting that nuclei play an important role in mechano-sensing of cells.

Research Products

• [Journal Article] Role of Nesprin-1 in Nuclear Deformation in Endothelial Cells under Static and Uniaxial Stretching Conditions (2012)
  • Author(s): T. Anno, N. Sakamoto, M. Sato
  • Journal Title: Biochemical and Biophysical Research Communications Volume: Vol.424 (1) Pages: 94-99
  • DOI: DOI:10.1016/j.bbrc.2012.06.073
  • Peer Reviewed
• [Presentation] Role of Nucleus-Actin Filament Binding in Endothelial Cell Responses to Cyclic Stretching (2013)
  • Author(s): N. Sakamoto, T. Anno, S. Chubachi, S.Deguchi, M. Sato
  • Organizer: International Symposium on Bio Medical Engineering Interface
  • Place of Presentation: 仙台
  • Year and Date: 2013-03-15
• [Presentation] 内皮細胞における細胞核とアクチンフィラメント結合の生体力学的検討 (2013)
  • Author(s): 坂元尚哉,阿武俊郎,中鉢辰也,出口真次,佐藤正明
  • Organizer: 日本生体医工学会専門別研究会バイオメカニクス研究会146回研究会
  • Place of Presentation: 徳島
  • Year and Date: 2013-01-25
• [Presentation] 原子間力顕微鏡を用いた細胞核-アクチンフィラメント結合の評価 (2013)
  • Author(s): 中鉢辰也,阿武俊郎,坂元尚哉,出口真次,佐藤正明
  • Organizer: 日本機械学会第25回バイオエンジニアリング講演会
  • Place of Presentation: つくば
  • Year and Date: 2013-01-10
• [Presentation] 細胞核ー細胞骨格結合が内皮細胞の力学応答に果たす役割 (2012)
  • Author(s): 中鉢辰也,阿武俊郎,坂元尚哉,出口真次,佐藤正明
  • Organizer: 第46回日本生体医工学会東北支部大会
  • Place of Presentation: 仙台
  • Year and Date: 2012-11-17
• [Presentation] Mechanical Role of Nesprin-1 in Nuclear Shape in Vascular Endothelial Cells Subjected to Stretching (2012)
  • Author(s): N. Sakamoto, T. Anno, M. Sato
  • Organizer: Biomedical Engineering Society2012 Annual Meeting
  • Place of Presentation: Atlanta (USA)
  • Year and Date: 2012-10-26
• [Remarks] 研究室ホームページ
  • URL: https://sites.google.com/site/sakamotonlab/