2012 Fiscal Year Final Research Report
Predicting the fate of lymphocytes by the initial Ca response pattern
| Project/Area Number |
23650258
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyoto University |
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Principal Investigator |
MATSUOKA Satoshi 京都大学, 大学院・医学研究科, 特定准教授 (00263096)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Ayako 京都大学, 大学院・医学研究科, 助教 (00378704)
KIM Bongju 京都大学, 大学院・医学研究科, 特定研究員 (80511823)
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| Project Period (FY) |
2011 – 2012
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| Keywords | リンパ球 / カルシウム / 抗原受容体 / 細胞死 / 細胞増殖 |
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| Research Abstract |
Lymphocyte cell lines (A20 B cells, etc.) and mouse spleen B lymphocytes were used for analyses. These cells were loaded with Ca2+fluorescent indicators (Fluo-4, etc.). Cytoplasmic Ca2+responses to the stimulation of surface antigen receptors were recorded from individual cells using a fluorescence microscope and the data were collected to make a database. Additionally, systems were developed to detect cell death, apoptosis and mitochondrial membrane potential from individual cells. Statistical analysis and correlational analysis were performed using these digitalized data.
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[Journal Article] Basophils are required for the induction of Th2 immunity to haptens and peptide antigens2013
Author(s)
Otsuka A, Nakajima S, Kubo M, Egawa G, Honda T, Kitoh A, Nomura T, Hanakawa S, Sagita Moniaga C, Kim B, Matsuoka S, Watanabe T, Miyachi Y, Kabashima K
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Journal Title
Nature Communications
Volume: 4
Pages: 1738
DOI
Peer Reviewed
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[Journal Article] Impairment of Ubiquitin-Proteasome System by E334K cMyBPC Modifies Channel Proteins, Leading to Electrophysiological Dysfunction2011
Author(s)
Bahrudin U, Morikawa K, Takeuchi A, Kurata Y, Miake J, Mizuta E, Adachi K, Higaki K, Yamamoto Y, Shirayoshi Y, Yoshida A, Kato M, Yamamoto K, Nanba E, Morisaki H, Morisaki T, Matsuoka S, Ninomiya H, Hisatome I.
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Journal Title
Journal of Molecular Biology
Volume: 413(4)
Pages: 857-78
DOI
Peer Reviewed
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[Journal Article] Requirement of Interaction between Mast Cells and Skin Dendritic Cells to Establish Contact Hypersensitivity2011
Author(s)
Otsuka A, Kubo M, Honda T, Egawa G, Nakajima S, Tanizaki H, Kim B, Matsuoka S,Watanabe T, Nakae S, Miyachi Y, Kabashima K
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Journal Title
PLoS One
Volume: 6(9)
Pages: e25538
DOI
Peer Reviewed
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[Journal Article] Minimum Information about a Cardiac Electrophysiology Experiment (MICEE): Standardised reporting for model reproducibility, interoperability, and data sharing2011
Author(s)
Quinn TA, Granite S, Allessie MA, Antzelevitch C, Bollensdorff C, Bub G, Burton RA, Cerbai E, Chen PS, Delmar M, Difrancesco D, Earm YE, Efimov IR, Egger M, Entcheva E, Fink M, Fischmeister R, Franz MR, Garny A, Giles WR, Hannes T, Harding SE, Hunter PJ
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Journal Title
Progress in Biophysics and Molecular Biology
Volume: 107(1)
Pages: 4-10
DOI
Peer Reviewed
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[Journal Article] Induction and enhancement of cardiac cell differentiation from mouse and human induced pluripotent stem cells with cyclosporin-a2011
Author(s)
Fujiwara M, Yan P, Otsuji TG, Narazaki G, Uosaki H, Fukushima H, Kuwahara K, Harada M, Matsuda H, Matsuoka S, Okita K, Takahashi K, Nakagawa M, Ikeda T, Sakata R, Mummery CL, Nakatsuji N, Yamanaka S, Nakao K, Yamashita JK
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Journal Title
PLoS One
Volume: 6(2)
Pages: e16734.
DOI
Peer Reviewed
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