2012 Fiscal Year Final Research Report
Development ofgenetically modified hepatic cells with induced high liver functions for bioartificial liver systems
| Project/Area Number |
23650289
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MIZUMOTO Hiroshi 九州大学, 大学院・工学研究院, 准教授 (90346817)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 生物・生体工学 / バイオテクノロジー / 発現制御 / 肝特異的転写因子 / バイオ人工肝臓 / ヘパトーマ |
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| Research Abstract |
Here, we attempted to create genetically engineeredhepatic cells with enhanced liver functions by overexpression of liver-enrichedtranscription factors (LETFs), which are associated with the transcription of liver-specific genes and hepatic differentiation. Mouse hepatoma Hepa1-6 cells were transduced with retroviral vectors, in which inducible expression cassettes for the LETF genes were introduced. Cell clones with inducible expression of high liver functions were established. This approach may be promising for the construction of cells for use in bioartificial liver systems.
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