Establishment of screening system for the candidate compounds for anti-cancer drugs with iPS cell

2013 Fiscal Year Final Research Report

• Project/Area Number: 23650587
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Carcinogenesis
• Research Institution: Tohoku University
• Principal Investigator: FUKUDA Tomokazu 東北大学, (連合)農学研究科(研究院), 准教授 (40321640)
• Project Period (FY): 2011 – 2012
• Keywords: エピジェネティクス

Research Abstract

We focused on the common biological feature between cancer and iPS cell, and tried to establish the screening system for de-methylating agents for the genome. We introduced reprogramming four factors into E6/E7 immortalized mouse embryonic fibroblasts (MEF). The infected MEF showed iPS transformation. We successfully established mouse iPS cells, and found that reported activity is dramatically dropped down. We are trying to screen the mow molecular compounds, which causes the re-activation of the promoter activity.

Research Products

• [Journal Article] Low-molecular-weight inhibitors of cell differentiation enable efficient growth of mouse iPS cells under feeder-free conditions (2014)
  • Author(s): Donai K, Inagaki A, So KH, Kuroda K, Sone H, Kobayashi M, Nishimori K, Fukuda T
  • Journal Title: Cytotechnology Volume: (in press)
  • DOI: 10.1007/s10616-013-9686-8
  • Peer Reviewed
• [Journal Article] Establishment of a reporter system to monitor silencing status in induced pluripotent stem cell lines (2013)
  • Author(s): Donai K, Kuroda K, Guo Y, So KH, Sone H, Kobayashi M, Nishimori K, Fukuda T
  • Journal Title: Anal Biochem Volume: 443 Pages: 104-112
  • DOI: 10.1016/j.ab.2013.08.014
  • Peer Reviewed
• [Presentation] 人工多能性幹細胞におけるLTRプロモーターのサイレンシング状態を培養上清から簡便にモニタリングできるレポーターシステムの樹立 (2013)
  • Author(s): 土内憲一郎, 黒田健吾, 郭熠洁, 蘇敬夏, 小林正之, 西森克彦, 福田智一
  • Organizer: 日本繁殖生物学会総会
  • Place of Presentation: 東京農工大学
  • Year and Date: 20130912-14
• [Presentation] Establishment of induced pluripotent stem cell lines that allow monitoring of the re-programming status of genomes (2012)
  • Author(s): Donai K., Kuroda K., So KH., Inagaki A., Nishimori K., Fukuda T
  • Organizer: Annual and International Meeting of the Japanese Association for Animal Cell Technology
  • Place of Presentation: Nagoya
  • Year and Date: 20121127-30
• [Presentation] Application of induced pluripotent stem cells for the screening of low molecular demethylating compounds (2012)
  • Author(s): Fukuda T
  • Organizer: Annual and International Meeting of the Japanese Association for Animal Cell Technology
  • Place of Presentation: Nagoya
  • Year and Date: 20121127-30