2012 Fiscal Year Final Research Report

Development of cancer stem cell (CSC) culturing system and identification of CSC-specific marker

Research Project

Project/Area Number	23650597
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Tumor biology
Research Institution	Tottori University
Principal Investigator	OSAKI Mitsuhiko 鳥取大学, 医学部, 准教授 (40325006)
Co-Investigator(Renkei-kenkyūsha)	YUGE Rui 広島大学, 保健学研究科, 教授 (20263676) KAWAHARA Yumi 広島大学, 保健学研究科, 研究員 (20457279)
Research Collaborator	ITO Hisao 鳥取大学, 理事 (60127610)
Project Period (FY)	2011 – 2012
Keywords	幹細胞
Research Abstract	We cultured cancer cells with high drug-resistant ability, using a three-dimentional (3D) clinostat, a device which produces an environment with an average of 10-3G over the time, as a culture method possibly capable of maintaining stemness of cancer stem cells. By culturing in the microgravity environment, cell growth was promoted compared to that in the normal gravity environment. We inoculated 1ｘ102～103cells cultured in the environment subcutaneously in nude mice, and found no tumorigenicity maintained, indicating that the culture under the microgravity environment was not sufficient to maintain highly purified cancer stem cells.