2012 Fiscal Year Final Research Report
Development of an innovative method for evaluating DDS formulationpharmacokinetics by using multi-photon excitation microscopy
| Project/Area Number |
23650621
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Clinical oncology
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| Research Institution | Tohoku University |
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Principal Investigator |
OUCHI Noriaki 東北大学, 大学院・医学系研究科, 教授 (90203710)
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| Co-Investigator(Kenkyū-buntansha) |
GONDA Kosuke 東北大学, 大学院・医学系研究科, 准教授 (80375435)
AMARI Masakazu 東北大学, 大学院・医学系研究科, 准教授 (50400312)
TADA Hiroshi 東北大学, 大学院・医学系研究科, 助教 (50436127)
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| Project Period (FY) |
2011 – 2012
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| Keywords | ドラッグデリバリー |
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| Research Abstract |
We prepared GFP-fused EB1-expressing human breast cancer cells. The growth rate of the microtubule plus-end was measured using the positions of EB1 assembly over time. Analysis of the dynamics of the EB1-GFP comet showed that the microtubule growth rates decreased when the cells were treated with anticancer drugs. We also measured the microtubule growth rates in tumor-bearing mice by using an original stereotaxic instrument. Thus, alteration in EB1-GFP dynamics is an effective indicator for the effects of anticancer drugs.
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