A method for observation of multi-cellular mechanics by fluorescent microscopy

2012 Fiscal Year Final Research Report

• Project/Area Number: 23651099
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Hokkaido University
• Principal Investigator: KAWABATA Kazushige 北海道大学, 大学院・先端生命科学研究院, 教授 (20261274)
• Co-Investigator(Kenkyū-buntansha): MIZUTANI Takeomi 北海道大学, 大学院・先端生命科学研究院, 助教 (40451405)
• Project Period (FY): 2011 – 2012
• Keywords: イメージング / 力学測定 / 細胞集団

Research Abstract

We tried to establish methods to measure strain within cytoskeletons or strain between cells. These methods will help us to study mechanical interaction between cytoskeletons or multi-cellular movement.

Research Products

• [Journal Article] Tempo-Spatial Change of Cellular Stiffness and Geometry in the Process of Developing Epithelial Cell-Cell Adhesion Measured by Atomic Force Microscopy (2013)
  • Author(s): R. Tanaka, T. Mizutani, H. Haga, K. Kawabata
  • Journal Title: Japanese Journal of Applied Physics Volume: (in press)
  • Peer Reviewed
• [Journal Article] Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain (2013)
  • Author(s): S. Ishihara, M. Yasuda, T. Nishioka, T. Mizutani, K. Kawabata, H. Shirato, H. Haga
  • Journal Title: FEBS Letter Volume: Vol. 587(6) Pages: 732-6
  • DOI: DOI:10.1016/j.febslet.2013.01.055
  • Peer Reviewed
• [Journal Article] The number of cyclic stretch regulates cellular elasticity in C2C12 myoblasts (2012)
  • Author(s): K. Takemoto, T. Mizutani, K. Tamura, K. Takeda, H. Haga, K. Kawabata
  • Journal Title: CellBio Volume: Vol.1(1) Pages: 1-10
  • DOI: DOI:10.4236/cellbio.2012.11001
  • Peer Reviewed
• [Journal Article] Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice (2012)
  • Author(s): N. Seito, T. Yamashita, Y. Tsukuda, Y. Matsui, A. Urita, T. Onodera, T. Mizutani, H. Haga, N. Fujitani, Y. Shinohara, A. Minami, N. Iwasaki
  • Journal Title: Arthritis & Rheumatism Volume: Vol. 64(8) Pages: 2579-88
  • DOI: DOI:10.1002/art.34463.
  • Peer Reviewed
• [Journal Article] 2次元と3次元環境下での細胞と細胞骨格の力学計測 (2012)
  • Author(s): 水谷武臣
  • Journal Title: M&M2012材料力学カンファレンス論文集
• [Journal Article] Active fluctuation in the cortical cytoskeleton observed by high-speed live-cell scanning probe microscopy (2011)
  • Author(s): K. Tamura, T. Mizutani, H. Haga, K. Kawabata
  • Journal Title: Acta Biomaterialia Volume: Vol. 7(10) Pages: 3766-3772
  • DOI: DOI:10.1016/j.actbio.2011.06.013.
  • Peer Reviewed
• [Presentation] Tempo-spatial Change of Cellular Stiffness in the Process of Developing Epithelial Cell-cell Adhesion Measured by Atomic Force Microscopy (2012)
  • Author(s): R. Tanaka, T. Mizutani, H. Haga, K. Kawabata
  • Organizer: 20th International Colloquium on Scanning Probe Microscopy
  • Place of Presentation: Okinawa Kariyushi Urban Resort Naha(那覇市)
  • Year and Date: 2012-12-17
• [Presentation] 2次元と3次元環境下での細胞と細胞骨格の力学計測 (2012)
  • Author(s): 水谷武臣
  • Organizer: M&M2012材料力学カンファレンス
  • Place of Presentation: 愛媛大学(松山市).
  • Year and Date: 2012-09-22
• [Presentation] Evaluation of microtubule deformation by live cell imaging and image analysis (2012)
  • Author(s): R. Tanaka, T. Mizutani, H. Haga, K. Kawabata
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学(名古屋市).
  • Year and Date: 2012-09-22
• [Presentation] 画像解析を用いた細胞内外の力学量計測 (2012)
  • Author(s): 水谷武臣
  • Organizer: 第四回「光塾」
  • Place of Presentation: 北海道大学(札幌市).
  • Year and Date: 2012-08-26
• [Presentation] 細胞骨格に発生した不均一な歪み:ライブセルイメージングと画像解析による力学量測定法の提案 (2012)
  • Author(s): 田中良昌、水谷武臣、芳賀永、川端和重
  • Organizer: 2011年度日本生物物理学会北海道支部例会
  • Place of Presentation: 旭川市民文化会館(旭川市)
  • Year and Date: 2012-03-06
• [Presentation] Imaging for Cellular Mechanics: Dynamic Behavior of Cytoskeletal Networks and Cellular Contractile Force (2011)
  • Author(s): T. Mizutani
  • Organizer: The Fifth Shanghai International Conference on Biophysics and Molecular Biology
  • Place of Presentation: Huashia Hotel (China)
  • Year and Date: 2011-11-05
• [Presentation] 細胞の時空間ダイナミクス計測:ライブセルイメージングから細胞骨格の力学バランスと細胞が発生する力をみる (2011)
  • Author(s): 水谷武臣
  • Organizer: 九州大学共同利用研究会
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2011-09-21
• [Remarks]
  • URL: http://altair.sci.hokudai.ac.jp/g3/index.html