2013 Fiscal Year Final Research Report
Studies on features and frequencies of recombination in bacterial evolution
| Project/Area Number |
23651183
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Genome biology
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | recombination / ShortReadManager / illumina |
|---|
| Research Abstract |
To clarify features and frequencies of bacterial recombinational events, two closely related bacterial strains were mixed and incubated in a sterile soil sample. After a long period of incubation cells were recovered on a solid medium, and subjected to illumina sequencing. The illumina sequencing reads were analyzed by developing and using a computational tool named 'ShortReadManager', which is now released at http://www.ige.tohoku.ac.jp/joho/gf/index.php, and I found no evidence that indicates recombination between the two strains.
On the other hand, mutated DNA fragment of 16S ribosomal RNA gene was inserted into a genome of a soil bacteria, in which 5 copies of rRNA gene are present. The mutated fragment was expected to be replaced by the wild-type copy. By quantitative PCR analysis, we found a evidence that suggests concerted evolution of multi gene family.
|
Research Products
(26 results)
-
-
[Journal Article] Complete Genome Sequence of the Thermophilic Polychlorinated Biphenyl Degrader Geobacillus sp. Strain JF8 (NBRC 109937)2014
Author(s)
Shintani M, Ohtsubo Y, Fukuda K, Hosoyama A, Ohji S, Yamazoe A, Fujita N, Nagata Y, Tsuda M, Hatta T, Kimbara K
-
Journal Title
Genome announcements
Volume: 2
Pages: e01213-13
DOI
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Genomic organization and genomic structural rearrangements of SphingobiumjaponicumUT26, an archetypal gamma- hexachlorocyclohexane- degrading bacterium2011
Author(s)
Nagata, Y., S. Natsui, R. Endo, Y. Ohtsubo, N. Ichikawa, A. Ankai, A. Oguchi, S. Fukui, N. Fujita, and M. Tsuda
-
Journal Title
Enzyme and Microbial Technology
Volume: 49
Pages: 499-508
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
