2013 Fiscal Year Final Research Report
Studies on features and frequencies of recombination in bacterial evolution
| Project/Area Number |
23651183
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | recombination / ShortReadManager / illumina |
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| Research Abstract |
To clarify features and frequencies of bacterial recombinational events, two closely related bacterial strains were mixed and incubated in a sterile soil sample. After a long period of incubation cells were recovered on a solid medium, and subjected to illumina sequencing. The illumina sequencing reads were analyzed by developing and using a computational tool named 'ShortReadManager', which is now released at http://www.ige.tohoku.ac.jp/joho/gf/index.php, and I found no evidence that indicates recombination between the two strains.
On the other hand, mutated DNA fragment of 16S ribosomal RNA gene was inserted into a genome of a soil bacteria, in which 5 copies of rRNA gene are present. The mutated fragment was expected to be replaced by the wild-type copy. By quantitative PCR analysis, we found a evidence that suggests concerted evolution of multi gene family.
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DOI
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