2013 Fiscal Year Final Research Report
Electrochemical regulation of nucleic acids hybridization
| Project/Area Number |
23655068
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OBIKA Satoshi 大阪大学, 大学院・薬学研究科, 教授 (80243252)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 生体分析 / 人工核酸 / 電気化学 / 電極修飾 / DNAセンサー / 電気化学的制御 / DNAコンジュゲート |
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| Research Abstract |
I have been trying to immobilize modified DNA carrying disulfide-bridged deoxyribose (ssDNA) and two terpyridine units (terpy2DNA) on carbon and gold electrode, respectively. I expect that I can monitor the hybridization by the change in their electrochemical properties.
ssDNA was immobilized on HPOG electrode through stacking interaction of aromatic molecules such as pyrene and perylene onto the HOPG. terpy2DNA was immobilized on Au electrode by using specific affinity of sulfhydryl group, which was modified at the end of terpy2DNA, to Au. Unfortunately, the electrochemical signal of sulfhydryl/disulfide redox pair in ssDNA was not observed. The potential would be out of the measurement window. terpy2DNA formed intramolecular 1:2 complex with some divalent transition metal ions to form [Fe(terpy)2]2+. The redox profile of [Fe(terpy)2]2+/3+ on terpy2DNA was clearly observed on the Au electrode. The hybridization of terpy2DNA was monitored by frequency dependence on SWV measurement.
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[Journal Article] In Situ Oxygenous Functionalization of a Graphite Electrode for Enhanced Affinity toward Charged Species and a Reduced Graphene Oxide Mediator2014
Author(s)
M. R. Karim, Y. Ikeda, T. Ide, S. Sugimoto, K. Toda, Y. Kitamura, T. Ihara, T. Matsui, T. Taniguchi, M. Koinuma, Y. Matsumoto, S. Hayami
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Journal Title
New J. Chem
Volume: 38
Pages: 2120-2127
Peer Reviewed
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