2012 Fiscal Year Final Research Report
Protein interference in mammalian cells and analysis of biological functions of PDI and sEH
| Project/Area Number |
23655162
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kwansei Gakuin University |
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Principal Investigator |
IMAOKA Susumu 関西学院大学, 理工学部, 教授 (60145795)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJIWARA Shinsuke 関西学院大学, 理工学部, 教授 (90263219)
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| Project Period (FY) |
2011 – 2012
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| Keywords | ドメイン干渉 / タンパク質発現制御法 / プロテインジスルヒドイソメラーゼ / 可溶性エポキシドヒドロラーゼ / ほ乳細胞 |
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| Research Abstract |
Protein interference was found in E. coli. by prof. fujiwara. Protein interference is that suppression of protein by domain-domain interaction which destabilizes whole protein. In this study, protein interference was applied for suppression of protein in mammalian cells. We found first that b’ domain of PDI constituted of a, b, b’ , a’ and c domains interacted with a’. Overexpression of b’ but not b domain significantly reduced the native PDI in HEK 293 cells.
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