2013 Fiscal Year Final Research Report
Development of gene transfection and genetic analysis by Laser induced Emergent Stress Wave
| Project/Area Number |
23656275
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Control engineering
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| Research Institution | Kanazawa Institute of Technology |
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Principal Investigator |
KOGI Mieko 金沢工業大学, バイオ・化学部, 教授 (50410288)
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| Co-Investigator(Kenkyū-buntansha) |
TOKUNAGA Yoshiaki 金沢工業大学, 工学部, 教授 (00072174)
UCHIDA Eriko 国立医薬品食品衛生研究所, 遺伝子細胞医薬部, 第1室室長 (80176685)
AIZAWA Koji 金沢工業大学, 工学部, 教授 (40222450)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 応力波 / Nd:YAGレーザ / 遺伝子導入 |
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| Research Abstract |
The technology which introduces foreign genes into the human cell is the fundamental technology or gene therapy or regeneration medicine. In this study, we developed the gene introduction system using the Laser induced Emerged Stress Wave for any kind of the cell and high gene introduction efficiency. Q-switch Nd:YAG laser was used and, a solid element consisting of EPDM and polyethylene terephthalates were used as a stress wave outbreak element. The peak pressure level was necessary for the introduction of the foreign material more than 10MPa. We were able to obtain the division that showed a little less than 100% of introduction efficiency by irradiating a laser from the other side for an adhesion side of the cell.
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