Biodegradation of nylon and its recycle

2013 Fiscal Year Final Research Report

• Project/Area Number: 23656533
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: University of Hyogo
• Principal Investigator: NEGORO Seiji 兵庫県立大学, 工学(系)研究科(研究院), 教授 (90156159)
• Co-Investigator(Renkei-kenkyūsha): TAKEO Masahiro 兵庫県立大学, 大学院・工学研究科, 准教授 (40236443) ; KATO Dai-ichiro 兵庫県立大学, 大学院・工学研究科, 助教 (60423901)
• Project Period (FY): 2011 – 2013
• Keywords: ナイロン / 酵素 / 生分解 / 薄膜

Research Abstract

Nylon hydrolase degrades various aliphatic nylons, including nylon-6 and nylon-66. We synthesized a nylon-66 copolymer, in which a part of an adipoyl unit of nylon-66 was replaced with a succinyl unit by interfacial polymerization. To quantify the reaction rate of the enzymatic hydrolysis of nylons at the surface of solid polymers, we prepared a thin-layer of nylons on the bottom surface of each well in a polystyrene-based micro-assay plate.
More than 99% of the copolymer with thicknesses of 260 nm were converted to water-soluble oligomers by nylon hydrolase. The degradation rate of the thin-layered nylon-6 was similarly analyzed. We demonstrate that this assay enables a quantitative evaluation of the reaction rate of hydrolysis at the interface between the solid and aqueous phases and a quantitative comparison of the degradability for various polyamides.

Research Products

• [Journal Article] Enzymatic hydrolysis of nylons : quantification of the reaction rate of nylon hydrolase for thin-layered nylons (2014)
  • Author(s): Nagai, K. Iida, K. Shimizu, K. Kinugasa, R. Izumi, M. Kato, D. Takeo, M. Mochiji, K. & Negoro S
  • Journal Title: Appl. Microbiol. Biotechnol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Nylonoligomer hydrolase promoting cleavage reactions in unnatural amide compounds (2014)
  • Author(s): Kamiya, K., Baba, T., Boero, M., Matsui, T., Negoro, S., & Shigeta. Y
  • Journal Title: J. Phys. Chem. Lett Volume: 5 Pages: 1210-1216
  • DOI: 10.1021/jz500323y
  • Peer Reviewed
• [Journal Article] 高分子ナイロンを加水分解する酵素(NylC)の発見 (2014)
  • Author(s): 加藤太一郎, 武尾正弘, 根来誠司
  • Journal Title: 生物工学会誌「酵素の潜在能力を引き出す設計・戦略」 Volume: 92巻特集(掲載決定)
  • Peer Reviewed
• [Journal Article] Crystallization and X-ray diffraction analysis of nylon hydrolase (NylC) from Arthrobacter sp (2013)
  • Author(s): Nagai, K., Yasuhira, K., Tanaka, Y., Kato, D., Takeo, M., Higuchi, Y., Negoro, S., & Shibata N
  • Journal Title: KI72 Acta Cryst Volume: F69 Pages: 1151-1154
  • DOI: 10.1107/S1744309113024263
  • Peer Reviewed
• [Journal Article] 立体構造を基盤としたナイロン加水分解酵素の開発と耐熱化機構 (2013)
  • Author(s): 根来誠司, 永井圭介, 大島 祥平, 飯田一希, 加藤太一郎, 武尾正弘, 持地広造, 柴田直樹, 樋口芳樹
  • Journal Title: 酵素工学ニュース Volume: 69 Pages: 12-18
  • Peer Reviewed
• [Journal Article] Three-dimensional structure of nylon hydrolase and mechanism of nylon-6 hydrolysis (2012)
  • Author(s): Negoro, S., Shibata, N., Tanaka, Y., Yasuhira, K., Shibata, H., Hashimoto, H., Lee, Y-H., Oshima, S., Santa, R., Oshima, S., Mochiji, K., Goto, Y., Ikegami, T., Nagai, K., Kato, D., Takeo, M., & Higuchi, Y
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 5079-5090
  • DOI: 10.1074/jbc.M111.321992
  • Peer Reviewed
• [Journal Article] Crystallization and X-ray diffraction analysis of nylon oligomer hydrolase (NylC) from Agromyces sp. KY5R (2011)
  • Author(s): Yasuhira, K., Shibata, N., Tanaka, Y., Kumagai, N., Tanaka, Y., Nagai, K., Kato, D., Takeo, M., Negoro, S., Higuchi, Y
  • Journal Title: Acta Cryst Volume: F67 Pages: 892-895
  • DOI: 10.1107/S1744309111022858
  • Peer Reviewed
• [Journal Article] Molecular dynamics studies on the mutational structures of a nylon-6 byproductdegrading enzyme (2011)
  • Author(s): Baba, T., Kamiya K., Matsui, T., Shibata, N., Higuchi, Y., Kobayashi, T., Negoro, S., & Shigeta Y
  • Journal Title: Chem. Phys. Lett Volume: 507 Pages: 157-161
  • DOI: 10.1016/j.cplett.2011.03.046
  • Peer Reviewed
• [Presentation] 各種ナイロンの酵素的モノマー化のための条件検討 (2013)
  • Author(s): 飯田一希, 永井圭介, 加藤太一郎, 武尾正弘, 根来誠司
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2013-09-19
• [Presentation] 高解像度X 線結晶構造解析を基盤としたナイロン加水分解酵素(NylC)の耐熱化機構の解明 (2013)
  • Author(s): 衣笠 凌, 永井圭介, 三田隆二, 篠田昌宜, 柴田 直樹, 樋口芳樹, 李 映昊, 後藤祐児, 加藤太一郎, 武尾正弘, 根来誠司
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2013-09-19
• [Presentation] 分子動力学シミュレーションによるナイロン加水分解酵素(NylC)の自己分断機構の解析 (2013)
  • Author(s): 永井圭介, 大島祥平, 柴田直樹, 樋口芳樹, 馬場 剛史, 重田育照, 加藤太一郎, 武尾正弘, 根来誠司
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2013-09-19
• [Presentation] 66 ナイロンダイマーのNylB 変異酵素を用いた分解挙動の解析 (2013)
  • Author(s): 矢部勇貴, 西口拓紀, 石和久 健, 加藤太一郎, 武尾正弘, 根来誠司
  • Organizer: 第65回日本生物工学会大会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2013-09-19
• [Presentation] ナイロン加水分解酵素の分子設計と生分解性ポリアミド開発への展望 (2013)
  • Author(s): 根来誠司
  • Organizer: 2013年度日本農芸化学会大会
  • Place of Presentation: 東北大学・川内キャンパス
  • Year and Date: 2013-03-28
  • Invited
• [Remarks]
  • URL: http://www.eng.u-hyogo.ac.jp/msc/msc3/