2013 Fiscal Year Final Research Report

Establishment of an extracellular secretion pathway of flavonoids via a vesicle transport

Research Project

Project/Area Number	23657028
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Plant molecular biology/Plant physiology
Research Institution	Tohoku University
Principal Investigator	NAKAYAMA Toru 東北大学, 工学(系)研究科(研究院), 教授 (80268523)
Project Period (FY)	2011 – 2012
Keywords	イソフラボン / ダイズ / 小胞 / 分泌 / 輸送 / 培養細胞
Research Abstract	In this study, changes in the transcription levels of a gene coding for an isoflavone specific beta-glucosidase of soybean, GmICHG, in the individual organs of soybean seedlings (cv. Enrei) in response to microbial infection and abiotic stresses were analyzed. GmICHG was originally expressed in abundance only in the roots and at low levels only in the other organs. The transcription of GmICHG in the roots and other organs was suppressed upon infection of the roots by Phytophthora sojae. Upon wounding of the cotyledon, a transient long-distance up-regulation of GmICHG transcription in the roots was observed. Cultured cells of soybean (cv. Enrei) were established. The cells contained isoflavones whose composition was very similar to that of the seedling roots. A method for transforming the cultured cells by Agrobacterium method and an imaging system of intracellular flavonoids were also established.