Perturbation analysis of the 9+2 pattern of the ciliary/flagellar axoneme

2012 Fiscal Year Final Research Report

• Project/Area Number: 23657046
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: The University of Tokyo
• Principal Investigator: HIRNO Masafumi 東京大学, 大学院・理学系研究科, 准教授 (10212177)
• Project Period (FY): 2011 – 2012
• Keywords: 微細構造 / 微小管

Research Abstract

Almost all motile cilia and flagella have a characteristic structure called the “9+2”. However, it has not been rationally explained why the 9+2 pattern is necessary for the ciliary or flagellar motility. The aim of this study is to examine motilities of axonemes with variable numbers of the outer doublet microtubules produced by a Chlamydomonas mutant, bld12. We found several interesting features about interactions between projections in the axonemal structures. These findings are important to understand mechanisms of the assembly and function of the 9+2 structure.

Research Products

• [Journal Article] The role of retrograde intraflagellar transport in flagellar assembly and function (2012)
  • Author(s): Engel, B. D., Ishikawa, H., Wemmer, K. A., Geimer, S., Wakabayashi, K., Hirono, M., Craige, B., Pazour, G. j., Witman, G. B., Kamiya, R., Marshall, W. F.
  • Journal Title: J. CellBiol. Volume: 199 Pages: 151-167
  • DOI: DOI:10.1083/jcb.201206068
  • Peer Reviewed
• [Journal Article] Scaffolding function of the Chlamydomonas procentriole protein CRC70, a member of the conserved Cep70 family (2011)
  • Author(s): Shiratsuchi, G., Kamiya, R., and Hirono, M.
  • Journal Title: J. Cell Sci. Volume: 124 Pages: 2964-2975
  • DOI: DOI:10.1242/jcs.084715
  • Peer Reviewed
• [Journal Article] Structures of SAS-6 suggest its organization in centrioles (2011)
  • Author(s): van Breugel, M., Hirono, M., Andreeva, A., Yanagisawa, H. A., Yamaguchi, S., Nakazawa, Y., Morgner, N., Petrovich, M., Ebong, I. O., Robinson, C. V., Johnson, C. M., Veprintsev, D., Zuber, B.
  • Journal Title: Science. Volume: 331 Pages: 1196-1199
  • DOI: DOI:10.1126/science.1199325
  • Peer Reviewed
• [Presentation] Interaction between SAS-6 and Bld10p, cartwheel components that establish the ninefold symmetry of the centriole (2012)
  • Author(s): Noga, A., Yamaguchi, S., Kamiya, R., and Hirono, M.
  • Organizer: 第64回日本細胞生物学会大会
  • Place of Presentation: 神戸
  • Year and Date: 20120500
• [Presentation] Mechanism for establishing the "nine-ness" of the centriole structure" Symposium "Biomimetics focused on the life style of Protista (2011)
  • Author(s): Hirono, M.
  • Organizer: 第49会日本生物物理学会年会
  • Place of Presentation: 姫路
  • Year and Date: 20110900
• [Presentation] A self-organizing mechanism for assembly of the conserved9-fold symmetrical structure of the centriole. Symposium "Challenges for innovation on the basis of protozoan principles (2011)
  • Author(s): Hirono, M.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 20110900
• [Presentation] Afm1, a conserved component of the flagellar outer doublet microtubule, is required for generation of the proper asymmetrical waveforms (2011)
  • Author(s): Fujita, A., Yanagisawa, H., Kamiya, R., and Hirono, M.
  • Organizer: 第63回日本細胞生物学会
  • Place of Presentation: 札幌
  • Year and Date: 20110600
• [Presentation] A novel Chlamydomonas mutant, tpg2, reveals a conserved 177-kDa protein associated with TTLL9, an enzyme that catalyzes tubulinpolyglutamylation in the axoneme (2011)
  • Author(s): Kubo, T., Yanagisawa, H., Liu, Z., Hirono, M., and Kamiya, R.
  • Organizer: 第63回日本細胞生物学会
  • Place of Presentation: 札幌
  • Year and Date: 20110600
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/seiri/chlamy/lab.html