The influence of transcript localization on post-translational modification

2012 Fiscal Year Final Research Report

• Project/Area Number: 23657137
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Rikkyo University (2012); Keio University (2011)
• Principal Investigator: YAMAMOTO Miki 立教大学, 理学部, ポストドクトラルフェロー (40301783)
• Project Period (FY): 2011 – 2012
• Keywords: 細胞構造 / 機能 / GPIアンカー修飾

Research Abstract

Post-translational modification of protein is important in promoting proper function of protein. In this study, we investigated whether post-translational modification of protein is regulated by mRNA localization or not. Dally-like protein (Dlp) in Drosophila has GPI-anchor which is a type of post-translational modification. And dlp mRNA shows restricted distribution near nucleus in wing disc cells. We changed the localization of dlp mRNA by replacing 3’-noncoding region of dlp with that of wingless which contains apical localization signal. The products translated from mislocalized mRNA did not show proper function and were accumulated in apical region of cells, suggesting that mRNA localization is involved in protein function and probably post-translationalmodification.

Research Products

• [Journal Article] Cisterna-specific localization of glycosylation-related proteins to the Golgi apparatus. (2012)
  • Author(s): Yamamoto-Hino,M., Abe,M., Shibano,T., Setoguchi,Y., Awano,W., Ueda.R., Okano,H.and Goto,S. Cell Struct.
  • Journal Title: Funct Volume: 37 Pages: 55-63
  • Peer Reviewed
• [Journal Article] Identification of proteasome components required for apical localization of Chaoptin using functional genomics (2012)
  • Author(s): Yano,H., Yamamoto-Hino,M., Awano,W., Aoki- Kinoshita,K.F., Tsuda-Sakurai,K., Okano,H. and Goto,S.
  • Journal Title: J. Neurogenet. Volume: 26 Pages: 53-63
  • Peer Reviewed
• [Presentation] ショウジョウバエゴルジ体に局在する Golgi complex-relatedglycoprotein1の同定と自然免疫における機能 (2013)
  • Author(s): 山本(日野)美紀
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: 名古屋ウインク愛知
  • Year and Date: 2013-06-19
• [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity (2012)
  • Author(s): 山本(日野)美紀
  • Organizer: International Endotoxin and Innate Immunity Society Meeting
  • Place of Presentation: 東京National Center ofSciences Building
  • Year and Date: 2012-10-24
• [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity (2012)
  • Author(s): 山本(日野)美紀
  • Organizer: 第10回日本ショウジョウバエ研究会
  • Place of Presentation: 東京慈恵医大
  • Year and Date: 2012-10-13
• [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity (2012)
  • Author(s): 山本(日野)美紀
  • Organizer: 第45回日本発生生物学会、第64回日本細胞生物学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2012-05-31
• [Presentation] Novel Roles ofGlycosylation in Drosophila InnateImmunity (2011)
  • Author(s): 山本(日野)美紀
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 神奈川横浜パシフィコ
  • Year and Date: 2011-12-14
• [Presentation] 自然免疫を制御する糖鎖修飾関連分子Senjuの解析 (2011)
  • Author(s): 山本(日野)美紀
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉幕張メッセ
  • Year and Date: 2011-11-27
• [Presentation] 自然免疫を制御する糖鎖修飾関連分子Senjuの解析 (2011)
  • Author(s): 山本(日野)美紀
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都京都国際会議場
  • Year and Date: 2011-09-24
• [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity (2011)
  • Author(s): 山本(日野)美紀
  • Organizer: 1 st Asia-Pacific Drosophila Research Conference
  • Place of Presentation: Chientan Youth Activity Center台湾
  • Year and Date: 2011-05-23
• [Book] Structure, Function and Formation of Glycans in Drosophila. In "Glycans: Biochemistry, Characterization and Applications. (2012)
  • Author(s): Yamamoto-Hino,M., Okano,H., Kanie,O. and Goto,S.
  • Total Pages: 165-188
  • Publisher: Nova Science publishers, Inc., NY
• [Remarks]
  • URL: http://goto-lab.net/