Isolation of citrus self-incompatibility gene based on the gene expression profile

2013 Fiscal Year Final Research Report

• Project/Area Number: 23658033
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Horticulture/Landscape architecture
• Research Institution: Kagoshima University
• Principal Investigator: KUBO TATSUYA 鹿児島大学, 農学部, 准教授 (70359983)
• Project Period (FY): 2011 – 2013
• Keywords: カンキツ / 自家不和合性

Research Abstract

We performed gene expression subtraction of the difference in developmental stages of bud for the purpose of isolation of citrus self-incompatibility gene. As a result, citT209 was isolated by stylar specificity. Performing the function estimation, it was estimated be a Cu / Zn SOD. On the other hand, self-incompatibility of citrus was shown could be overcome by CuSO4 sprays to bud. In addition, it is enhanced by CuSO4 sprays to bud has revealed that the expression level of Cu / Zn SOD gene, which is an antioxidant enzyme. The pollen tube growth and germination of the pollen is inhibited by the active oxygen. Expression level of Cu / Zn SOD gene is increased by spraying CuSO4 to bud, as a result, was suggested that active oxygen is removed, overcoming the effects of incompatibility can be obtained.