2013 Fiscal Year Final Research Report
Establishment of conditional knockout animal model for analyzing the pathogenesis of follicular cyst in the goat.
| Project/Area Number |
23658223
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied animal science
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| Research Institution | Nagoya University |
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Principal Investigator |
OHKURA Satoshi 名古屋大学, 生命農学研究科, 教授 (20263163)
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| Co-Investigator(Renkei-kenkyūsha) |
FURUSAWA Tadashi 独立行政法人農業生物資源研究所, 動物科学研究領域, 研究員 (00343997)
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| Research Collaborator |
MATSUDA Fuko 名古屋大学, 大学院・生命農学研究科, 准教授 (10608855)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 応用動物 / 神経科学 / 獣医学 / 生理学 / 畜産学 / ヤギ / キスペプチン / 卵胞嚢腫 |
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| Research Abstract |
In the present study, as the initial step in establishing a region-specific, conditional KISS1 gene knockout goat, we first revealed the genomic sequence of goat KISS1 gene 5'-upstream region up to 3 kb from translation initiation site. This information can be used for further evaluation of the promoter activity of goat KISS1 gene expression and establishment of conditional knockout model. Second, we demonstrated that an intravenous infusion of estrogen induced surge-like luteinizing hormone (LH) release both in gonadectomized male and female goats, and kisspeptin neurons in the medial preoptic area (mPOA) were activated by estrogen. In castrated male goats, mean peak LH concentration was lower and mean peak time of the LH surge was later than females. These results suggest that deterioration in functioning of mPOA kisspeptin neurons in response to estrogen in male goats can be applied as a model for analyzing the neuronal pathogenesis of follicular cyst in female ruminants.
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[Journal Article] Molecular cloning and identification of transcriptional regulatory domain of the goat neurokinin B gene TAC32013
Author(s)
Suetomi, Y., Matsuda, F., Uenoyama, Y., Maeda, K.-I., Tsukamura, H. and Ohkura, S
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Journal Title
Journal of Reproduction and Development
Volume: 59
Pages: 463-469
DOI
Peer Reviewed
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[Journal Article] Effects of full-length kisspeptin administration on follicular development in Japanese Black beef cows2013
Author(s)
Naniwa, Y., Nakatsukasa, K., Setsuda, S., Oishi, S., Fujii, N., Matsuda, F., Uenoyama, Y., Tsukamura, H., Maeda, K.-I. and Ohkura, S
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Journal Title
Journal of Reproduction and Development
Volume: 59
Pages: 588-594
DOI
Peer Reviewed
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[Journal Article] Differential effects of continuous exposure to the investigational metastin/kisspeptin analog TAK-683 on pulsatile and surge mode secretion of luteinizing hormone in ovariectomized goats2013
Author(s)
Tanaka, T., Ohkura, S., Wakabayashi, Y., Kuroiwa, T., Nagai, K., Endo, N., Tanaka, A., Matsui, H., Kusaka, M. and Okamura, H
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Journal Title
Journal of Reproduction and Development
Volume: 59
Pages: 563-568
DOI
Peer Reviewed
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[Book] Chapter 14: Kisspeptin and GnRH pulse generation. In Kisspeptin Signaling in Reproductive Biology(Eds. A.S. Kauffman and J.T.Smith.)2013
Author(s)
Okamura, H., Tsukamura, H., Ohkura, S., Uenoyama, Y., Wakabayashi, Y. and Maeda, K.-I
Total Pages
297-324
Publisher
Springer, Berlin
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